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Updated: Sep 17, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
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在活细胞和多细胞生物体中单分子追踪和动态分析

J Christof M Gebhardt1

  • 1Institute of Experimental Physics and IQST, Ulm University, Ulm, Germany.

Journal of molecular biology
|June 27, 2025
PubMed
概括

单分子追踪 (SMT) 可视化活细胞中的分子动态. 本综述详细介绍了SMT方法用于研究分子动力学,特别是转录因子,从而促进生物过程的理解.

关键词:
光片微观镜片的光片微观镜片活细胞成像技术使用.活细胞单分子追踪分子运动分析 分子运动分析单分子局部化显微镜.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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