通过纤维素酵母发酵克服葡萄糖抑制,可以增强工程化Saccharomyces cerevisiae中富含点氨酸的生物质的生物转化
Dahye Lee1, Deokyeol Jeong1, Soo Rin Kim2
1Department of Food Science, Purdue University, West Lafayette, IN 47907, USA; Whistler Center for Carbohydrate Research, Purdue University, West Lafayette, IN 47907, USA.
Bioresource technology
|June 27, 2025
概括
工程酵母在低pH值下高效地发酵富含点氨酸的生物质,同时利用纤维素,西洛和银酸. 这种强大的平台增加了乳酸生产,提供了一种可持续的废物利用方法.
科学领域:
- 生物技术是生物技术.
- 微生物工程 微生物工程
- 工业微生物学 工业微生物学
背景情况:
- 富含点的生物质是发酵的宝贵原料.
- 在Saccharomyces cerevisiae中,低pH值和葡萄糖抑制阻碍了同时利用糖,特别是银酸.
- 开发强大的微生物菌株以有效降解和发酵pectin对于可持续的化学生产至关重要.
研究的目的:
- 在酸性条件下设计Saccharomyces cerevisiae,用于在酸性条件下共同发酵纤维素,纤维素和银酸.
- 为了克服低pH值和葡萄糖抑制的局限性,以提高素衍生糖的利用率.
- 开发一个微生物平台,从富含点氨酸的废物中生产增值化学品.
主要方法:
- 适应性实验室进化Saccharomyces cerevisiae以提高耐受性和基质利用在低pH (pH3.5) 的情况下.
- 基因分析以识别带来性能改善的突变,特别是细胞德克斯林载体 (M128I和L167H突变) 中的突变.
- 设计用于乳酸生产的进化菌株,以评估其用于化学合成的潜力.
主要成果:
- 进化的菌株 (DH1) 显著增强了蜂菌的摄取量 (增加了6倍),并改善了银酸的利用率 (约1. 在pH值3.5时增加2倍).
- 用蜂糖替代葡萄糖减轻了葡萄糖抑制,增强了细胞内NADPH,并使混合糖的有效共用成为可能.
- 改造品种的乳酸位达到38.2g/L,与糖养对照群相比,改善了46.4%.
结论:
- 在适应性实验室进化中,成功生成了一种Saccharomyces cerevisiae菌株,能够在具有工业意义的酸性条件下共同发酵关键的点丁衍生糖.
- 细胞德克斯林载体中发现的突变对于增强基质吸收和耐受性至关重要.
- 这种工程微生物平台展示了可持续生物转化富含点氨酸的废物到有价值的化学物质,如乳酸的显著潜力.
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