在复杂和无异生菌介质中培养的口腔粘膜上皮细胞中的DNA损伤:一项比较研究
Joao Victor Cabral1, Sona Vodenkova2,3, Kristyna Tomasova2,3
1Laboratory of Biology and Pathology of the Eye, Institute of Biology and Medical Genetics, 1st Faculty of Medicine and General Teaching Hospital, Charles University, Czech Republic.
Mutagenesis
|June 28, 2025
概括
口腔粘膜上皮细胞 (OMECs) 在复杂和无异生菌介质中都表现出基因组稳定性,支持它们在肢体干细胞缺乏症治疗中的使用. 无异生菌介质为眼皮表面移植提供了更安全的临床选择.
科学领域:
- 眼科医生 眼科 眼科
- 细胞生物学 细胞生物学
- 再生医学是一种再生医学.
背景情况:
- 口腔粘膜上皮细胞 (OMEC) 是治疗肢体干细胞缺乏症 (LSCD) 的潜在自身细胞来源.
- 确保培养细胞的基因组稳定性对于成功的眼皮表面移植至关重要.
- 对比复杂介质 (COM) 和无异生菌介质 (XF) 对临床应用的安全性至关重要.
研究的目的:
- 评估在COM与XF介质中培养的OMECs的基因组稳定性.
- 评估这些培养条件对于潜在的LSCD治疗的适用性.
- 为了确定XF介质是否具有较低的DNA损伤风险,用于临床使用.
主要方法:
- 进行微核 (MNi) 测试以评估染色体损伤.
- 彗星测试被用来检测DNA链断裂和氧化病变.
- 细胞形态,活力和大小在COM和XF培养之间进行了比较.
主要成果:
- 在COM和XF组之间没有观察到细胞形态,活力或大小的显著差异.
- 微核频率相似 (COM中的5.67MNi/1000细胞,XF中的6.17MNi/1000细胞).
- 彗星测定显示,在两种介质中,DNA损伤水平较低,XF介质的损伤趋势较小.
结论:
- 可以在COM和XF介质中培养OMEC,而不需要显著的基因组不稳定性.
- 无异生菌介质 (XF) 是对OMECs进行临床培养的可行和潜在的更安全的选择.
- 这些发现支持OMEC用于眼表面移植的使用,优化培养条件以确保安全性和有效性.
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