表面温度变化表明肺小鼠冠状病毒感染后的疾病发病,但不构成人类终点
Rebecca Nistelberger1, Patrizia Gibler2, Thomas Filip3
1Core Facility Animal Breeding and Husbandry, Medical University of Vienna, Vienna, Austria.
Veterinary research communications
|June 28, 2025
概括
表面温度不是在小鼠冠状病毒感染期间动物福利的可靠指标. 体重变化是确定感染小鼠的人性终点的更好的标志物.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 动物模型 动物模型
背景情况:
- 鼠标模型对于研究感染期间的免疫系统至关重要.
- 需要客观的参数来监测感染的进展,并确保动物福利.
研究的目的:
- 评估通过热成像测量的背部表面温度是否可以作为在小鼠冠状病毒 (MCoV) 感染期间动物福祉的标志物.
- 为了比较表面温度与体重变化的实用性,在评估疾病严重程度和确定人类终点时.
主要方法:
- 在BALB/c和C57BL/6小鼠中感染了MCoV.
- 使用热成像监测背部表面温度.
- 定期测量体重以追踪体重减轻.
主要成果:
- 受感染的BALB/c小鼠表现出严重的症状,体温略有升高,但体重显著下降.
- 在C57BL/6小鼠中,症状不那么严重,体温升高,体重减轻显著.
- 个体动物的温度和体重变化没有相关性.
- 体重总是表明疾病的进展,并且在很大一部分小鼠中达到人性化的终点.
结论:
- 表面温度不是MCoV感染小鼠的人性终点的合适预测参数.
- 体重是评估疾病严重程度和确定早期终止点的更可靠指标.
- 表面温度可能表明肺病毒感染的发作,但不能表明动物的福祉.
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