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在沉浸式和固态发酵下,转录基因分析脂质积累在 Mucor circinelloides WJ11 中

Bushra Iqbal¹, Muhammad Tariq Saeed^1,2, Tahira Naz^1,3

¹Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255000, China.

Applied biochemistry and biotechnology

|June 28, 2025

概括

固态发酵 (SSF) 与浸泡发酵 (SmF) 相比,从Mucor circinelloides中产生的生物油较少. 转录基因分析显示,SSF中的脂肪酸合成途径减少,这解释了这种经济高效的方法中较低的脂质含量.

科学领域:

生物技术是生物技术.
菌类学菌类学是指菌类学.
代谢工程是代谢工程.

背景情况:

油性真菌对生物油生产具有前景.
浸泡发酵 (SmF) 和固态发酵 (SSF) 是关键的技术.
粘菌 (Mucor circinelloides) 是一种研究脂质积累的真菌物种.

研究的目的:

为了比较M. circinelloides中使用SmF和SSF的生物油生产.
研究SMF和SSF之间的脂肪酸生物合成差异背后的分子机制.
为了确定关键的基因和受SSF影响的途径在M. circinelloides WJ11中.

主要方法:

在SmF和SSF条件下种植M.circinelloides菌株WJ11.
脂肪酸 (FA) 含量的量测定.
转录组分析用于比较SmF和SSF之间的基因表达特征.

主要成果:

120小时后,SSF导致FA含量显著降低 (12%) 与SmF (24.1%) 相比.
转录组分析揭示了7615个基因的差异表达,其中关键基因在甘油三合成和乙-CoA生产下调在SSF.
低调酸通路 (PPP) 和依赖NADP+的酶活性在SSF中的下调减少了FA生物合成的NADPH供应.

结论:

固态发酵导致M. circinelloides WJ11中的脂质积累减少,与浸泡发酵相比.
转录组数据强调了关键路径的下调,包括糖脂代谢和脂肪酸生物合成,这是SSF中FA含量较低的主要原因.
了解这些分子差异可以指导通过真菌发酵优化生物油生产的战略.

关键词:

粘菌是循环类的.脂质生物合成脂质生物合成这是SSF的SSF.在 SmF SmF 中.文字转录学 (Transcriptomics) 是一个学科.

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