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PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

Overview
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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基于探针的qPCR工作流程使用快速核酸采样和分子基因定型在双重植物中的分子基因定型.

Anthony Torres1, Reginald Gaudino1,2

  • 1Cannabis Research Institute, Discovery Partners Institute, University of Illinois System, Chicago, IL, USA.

Methods in molecular biology (Clifton, N.J.)
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PubMed
概括

本研究详细介绍了一种基于探针的定量聚合酶链反应 (qPCR) 方法,用于植物基因定型. 该协议有效地识别来自各种DNA来源的男性特异性基因型,包括原始提取物.

关键词:
阿姆普利康 (Amplicon) 是一个周期门是指周期的门值.它们是DNA DNA DNA DNA.检测 检测 检测 检测 检测美国自由贸易协会的卡片.基因型定制是指基因型定制.男性特异性基因型的基因型.这是一个PCRPCR.植物样本 植物样本在预放大时,预放大.预放大增强方法的丰富方法它们是初级的.探测器探测器探测器基于探测器的探测方法qPCRR 是一个很好的方法.qPCRR 是一个很好的方法.

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科学领域:

  • 植物遗传学 植物遗传学
  • 分子生物学分子生物学
  • 生物技术是生物技术.

背景情况:

  • 精确的植物基因型定型对于育种和研究至关重要.
  • 传统的方法可能是劳动密集型的,需要高纯度的DNA.
  • 需要高吞吐量,可适应各种样本类型的特定基因造型.

研究的目的:

  • 为基于探针的定量聚合酶链反应 (qPCR) 基因定型提供一个强大的协议.
  • 为了在植物样本中实现雄性特异性基因型识别.
  • 用各种样本类型来证明该方法的有效性,包括原始提取物.

主要方法:

  • 从原始组织或FTA卡片中分离DNA.
  • 孤立DNA的预放大丰富反应.
  • 基于探头的定量聚合酶链反应 (qPCR) 用于基因型检测.

主要成果:

  • 在植物DNA中成功识别男性特异性基因型.
  • 实现了高特异性,用于对各种样本类型的基因型定型.
  • 这种方法即使使用原始制备的核酸提取物,也很强大.

结论:

  • 描述的基于探针的qPCR协议是植物基因定型的高通量和特定方法.
  • 它是有效的男性特异性基因型识别使用不同的DNA来源.
  • 该协议对原油提取物的适应性提高了其在植物科学中的实际应用.