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Updated: Jun 1, 2026

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
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基于探针的qPCR工作流程使用快速核酸采样和分子基因定型在双重植物中的分子基因定型

Anthony Torres1, Reginald Gaudino1,2

  • 1Cannabis Research Institute, Discovery Partners Institute, University of Illinois System, Chicago, IL, USA.

Methods in molecular biology (Clifton, N.J.)
|June 28, 2025
PubMed
概括

本研究详细介绍了一种基于探针的定量聚合酶链反应 (qPCR) 方法,用于植物基因定型. 该协议有效地识别来自各种DNA来源的男性特异性基因型,包括原始提取物.

关键词:
阿姆普利康 (Amplicon) 是一个周期门是指周期的门值.它们是DNA DNA DNA DNA.检测 检测 检测 检测 检测美国自由贸易协会的卡片.基因型定制是指基因型定制.男性特异性基因型的基因型.这是一个PCRPCR.植物样本 植物样本在预放大时,预放大.预放大增强方法的丰富方法它们是初级的.探测器探测器探测器基于探测器的探测方法qPCRR 是一个很好的方法.qPCRR 是一个很好的方法.

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PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

Overview
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...