通过桑格测序进行向的安普利康基因型鉴定
Anthony Torres1, Reginald Gaudino2,1
1Cannabis Research Institute, Discovery Partners Institute, University of Illinois System, Chicago, IL, USA.
Methods in molecular biology (Clifton, N.J.)
|June 28, 2025
概括
桑格测序是一种具有成本效益,准确的向DNA测序方法. 本协议详细介绍了原料设计,链终结,电泳和染色体分析,用于识别遗传变异.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物技术是生物技术.
背景情况:
- 桑格测序仍然是针对性DNA分析的宝贵工具.
- 传统方法提供了速度,成本效益和准确性的平衡.
研究的目的:
- 为了介绍执行桑格测序的详细协议.
- 概述准确的基准识别和变异检测的步骤.
主要方法:
- 设计用于DNA放大的特定原料.
- 包含标有光的,链末端的二氧化核酸.
- 通过电泳分离DNA碎片并分析色谱.
主要成果:
- 在DNA amplicon中精确的基因识别.
- 检测核酸变化表明遗传变异.
- 促进向基因中的氨基酸变异分析.
结论:
- 桑格测序为有针对性的基因分析提供了一种可靠的方法.
- 该协议可以准确识别核酸和氨基酸变异.
- 这种技术仍然适用于经济高效和快速的遗传研究.
相关概念视频
01:57Sanger Sequencing
757.9K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
757.9K
03:00Next-generation Sequencing
92.7K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
92.7K