相关实验视频

Updated: Sep 17, 2025

Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells

Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells

Published on: March 7, 2025

维斯塔免疫检查点使辐射疗法诱导的抗瘤免疫反应变得模糊

Dhanya K Nambiar¹, Sainiteesh Maddineni², Jimpi Langthasa¹

¹Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA, USA.

|June 28, 2025

概括

癌症的放射治疗可能由于免疫抑制性髓状细胞而失败. 在这些细胞上准V-域免疫球蛋白抑制T细胞激活 (VISTA) 增强了癌症治疗期间的瘤控制和免疫反应.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
放射治疗研究放射治疗研究

背景情况:

放射治疗 (RT) 对于治疗像头癌 (HNC) 这样的固体瘤至关重要.
矛盾的是,RT可以促进免疫抑制性髓状细胞,导致治疗耐药性.
在RT期间,V-域免疫球蛋白抑制T细胞激活 (VISTA) 在髓状细胞中的作用尚不清楚.

研究的目的:

在HNC的RT背景下,研究VISTA对髓状细胞的功能.
评估针对各种临床前癌症模型的VISTA结合RT的治疗潜力.

主要方法:

对小鼠和人类HNC瘤微环境 (TME) 的髓状细胞VISTA表达的分析.
在接受RT治疗的VISTA淘汰赛 (VISTA-/-) 小鼠中评估瘤控制和免疫反应.
在临床前癌症模型 (HNC,乳腺,结肠直肠) 中使用抗VISTA抗体和RT组合治疗的评估.
单细胞RNA测序 (sc-RNA-seq) 用于分析髓状细胞的特性.

主要成果:

在HNC TME的髓状细胞上观察到高VISTA表达,随着RT增加.
VISTA-/-小鼠通过RT增强了瘤控制,其特征是抗瘤起源的髓状细胞.
在多个模型中,抗VISTA抗体和RT的联合治疗显著降低了瘤体积.
联合治疗通过骨髓层再极化增强了全身抗瘤免疫力和瘤内T细胞功能.

结论:

骨髓细胞上的VISTA在固体瘤中对RT耐药性起着至关重要的作用.
针对VISTA是一个有希望的策略,可以克服RT诱导的免疫抑制.
将抗VISTA治疗与RT结合起来,可以显著提高治疗疗效和抗瘤免疫反应.

关键词:

CP: 癌症癌症癌症 CP: 免疫学免疫学在HNSCC中,我们可以看到HNSCC.在MDSC中,MDSC是MDSC.在视野中看到的.抗瘤免疫反应是一种反瘤免疫反应.免疫检查点是一个检查点.辐射疗法辐射疗法

更多相关视频

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Published on: June 12, 2021

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

Published on: April 22, 2019

相关概念视频

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...

Cancer Therapies

Cancer Therapies

Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...

Cancer Vaccines

Cancer Vaccines

Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...