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在神经顶早期发育过程中Pumilio基因表达的表征

Mariann Guzman-Espinoza¹, Helen M Vander Wende¹, Jessica L Pacheco²

¹Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, CA, USA; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA, USA.

Differentiation; research in biological diversity

|June 28, 2025

概括

Pumilio-1 (PUM1) 和Pumilio-2 (PUM2) RNA结合蛋白调节神经细胞的发育. 虽然两者都被广泛表达,但PUM1在神经的特异化和上皮-介质细胞过渡 (EMT) 过程中是均的,而PUM2在经历EMT的细胞中被丰富,这表明它们有不同的作用.

科学领域:

发展生物学发展生物学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

神经细胞对于脊椎动物的发育至关重要,形成多种细胞类型.
转录后调节对于神经峰的发展至关重要,但具体的调节者尚未完全理解.
米利奥蛋白是已知的RNA结合蛋白,参与转录后调节.

研究的目的:

识别和表征神经峰发育的转录后调节者.
调查Pumilio家族成员 (PUM1和PUM2) 在鸟类头骨神经发展中的作用.
确定神经峰发育期间PUM1和PUM2的时空表达模式.

主要方法:

单细胞RNA测序以确定候选调节剂.
杂交连锁反应 (HCR) 在Gallus gallus () 胚胎中.
对Pumilio家族mRNAs的时空表达分析.

主要成果:

PUM1和PUM2mRNA在早期胚胎组织中表达,在外皮衍生组织中进行丰富.
在特异化和上皮-介质细胞过渡 (EMT) 过程中,PUM1在神经管和神经峰中表现得均.
PUM2在准备EMT的神经细胞中特别丰富,表明了不同的表达模式.

结论:

PUM1和PUM2是神经顶部发育的候选后转录调节剂.
在神经的发育过程中,PUM1和PUM2表现出不同的时空表达模式.
假设PUM1和PUM2在EMT等关键神经发育事件中发挥非冗余的特定作用.

关键词:

一个漂亮的小.形态发生形态发生形态发生神经峰的神经峰在Pumilio Pumilio中,你会看到一些东西.配合RNA结合的蛋白质

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