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Updated: Sep 17, 2025

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
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替代结合作为重组蛋白净化潜在标签的替代结合

Richmond A Adomako1, Michael B Owusu1, Airam Cordova1

  • 1Department of Chemistry, East Texas A&M University, 2600 S Neal Street, Commerce, TX 75428, USA.

Journal of inorganic biochemistry
|June 28, 2025
PubMed
概括

一种新型的七分表明有效地与切拉树脂结合,为蛋白质净化提供了传统聚化标签的潜在替代品. 这种显示出强大的结合和有效的化,在生物化学净化工作流中很有用.

关键词:
固定化的金属亲和度色谱.标签是一种标签.重组蛋白质净化再组合蛋白质净化IMAC 的 IMAC 的 IMAC 的结合的类.类的复合体是类的复合体

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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