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从不同供体细胞来源克隆牛的基因组表观遗传差异的比较
Mingxuan Sheng1, Dongdong Bo2, Haoqiang Ma1
1Henan Province Livestock Genome Editing and Biobreeding Engineering Research Center, School of Life Sciences, Henan University, Kaifen, 475004, China.
Theriogenology
|June 28, 2025
概括
体细胞核转移 (SCNT) 的效率通过选择胎儿卵管上皮细胞 (FOV) 而不是胎儿纤维细胞 (FFB) 来提高. 来自FOV的克隆显示出更好的表观遗传重编程和基因表达,从而推进了牛克隆技术.
科学领域:
- 生殖生物学 生殖生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
背景情况:
- 体细胞核转移 (SCNT) 对畜牧养殖至关重要,但面临着低效率和发展问题的挑战.
- 捐赠细胞类型显著影响SCNT结果,但潜在的分子机制仍然不完全理解.
研究的目的:
- 研究由胎儿纤维细胞 (FFB) 和胎儿卵管上皮细胞 (FOV) 衍生出的牛克隆之间的表观遗传差异.
- 根据供体细胞来源,确定影响SCNT效率和发育成功的分子因素.
主要方法:
- 多omics分析,包括ATAC-seq,RNA-seq和DNA甲基化 (5mC) 和基甲基化 (5hmC) 分析.
- 系统地比较FFB和FOV衍生的SCNT克隆之间的表观遗传景观和基因表达模式.
- 综合性分析以确定差异化的基因组区域和转录因子结合动机.
主要成果:
- 与FFB衍生的克隆相比,FOV衍生的克隆显示出更好的染色质可访问性和基因表达.
- 观察到明显的表观遗传重编程动态,FOV克隆显示SCNT后5mC降低.
- 升高的5hmC水平与FFB克隆的生存相关,而已故的克隆的水平较低.
结论:
- 捐赠细胞选择通过不同的表观遗传重编程途径对SCNT结果产生关键影响.
- 与胎儿纤维细胞相比,胎儿卵管上皮细胞在牛中为SCNT提供了更有利的表观遗传特征.
- 这些发现为提高SCNT效率和牲畜发育成功提供了宝贵的见解.
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