一个染色体级参考基因组组合的吉尔伯特的皮肤Plestiodon gilberti
Jonathan Q Richmond1, Merly Escalona2, Mohan P A Marimuthu3
1U.S. Geological Survey, 4165 Spruance Rd. Suite 200, San Diego, CA 92101 United States.
The Journal of heredity
|June 28, 2025
概括
吉尔伯特皮 (Plestiodon gilberti) 的新染色体级基因组组合为了解爬行动物的物种化和通过基因流动的适应提供了关键资源. 这个参考基因组有助于对杂交研究的研究.
科学领域:
- 基因组学就是基因组学.
- 进化生物学 进化生物学
- 类学 类学 类学
背景情况:
- 基因流和杂交在物种进化中越来越被认可.
- 在大多数种群中,理解适应性内进仍然是一个挑战.
- 科家族包括许多物种,但缺乏广泛的基因组资源.
研究的目的:
- 为了生成一个高质量的,染色体水平的基因组组合的吉尔伯特的皮肤 (Plestiodon gilberti).
- 为加利福尼亚保护基因组学项目 (CCGP) 提供基础基因组学资源.
- 为了促进未来对Plestiodon属的物种化,杂交和适应性的研究.
主要方法:
- 利用太平洋生物科学HiFi长读取基因组测序.
- 使用的Omni-C近距离结合数据用于染色体支架.
- 执行了吉尔伯特的皮基因组的 de novo 组装和注释.
主要成果:
- 生成了一个1.57 Gb的染色体级组件,其支架N50为231.32 Mb.
- 实现了高组装完整性 (97.2%的BUSCO评分) 和读取覆盖范围 (~56X).
- 这代表了第一个北美皮肤参考基因组,也是全球少数几个基因组之一.
结论:
- 吉尔伯特的基因组组合是研究生态物种化的重要工具.
- 它将使研究能够研究血统分歧和杂交的遗传基础.
- 该资源支持研究如何内进可能驱动爬行动物的适应.
相关概念视频
Polytene Chromosomes
10.3K
Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also...
10.3K
Lampbrush Chromosomes
8.1K
In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
8.1K
Genome Annotation and Assembly
19.3K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
19.3K
Multi-species Conserved Sequences
4.3K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
4.3K
Karyotyping
62.5K
Overview
62.5K
Evolutionary Relationships through Genome Comparisons
6.2K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
6.2K


