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系统性硬化症中的抗线粒体抗体向肠道神经元,并与肠道不运动有关
Zsuzsanna H McMahan1, Srinivas N Puttapaka2, Livia Casciola-Rosen3
1Division of Rheumatology, UTHealth Houston, Houston, TX, USA.
在全身性硬化症 (SSc) 患者中,抗线粒体M2自身抗体 (AM2A) 与胃肠道过渡速度较慢相关. 这些自身抗体可以透到活细胞中,准线粒体,并可能导致SSc相关的肠胃不运动.
科学领域:
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
- 自免疫性疾病 自免疫性疾病
背景情况:
- 系统性硬化症 (SSc) 经常导致胃肠道 (GI) 功能障碍,与肠道神经系统 (ENS) 功能障碍有关.
- 一部分SSc患者具有抗线粒体M2自身抗体 (AM2A).
研究的目的:
- 为了确定M2抗原是否表达在特定的ENS细胞中.
- 调查SSc患者的AM2A和肠道不运动之间的关联.
主要方法:
- 从154名SSc患者的血清中使用ELISA选AM2A.
- 评估了GI过境,并将AM2A阳性和AM2A阴性组进行了比较.
- 用SSc血清培养ENS细胞和肝细胞癌细胞 (HepG2),并对AM2A进行染色.
主要成果:
- AM2A阳性 (12.9%) 与食道和胃运输速度减慢有显著联系.
- 免疫测试揭示了人类ENS神经元 (MENS) 中的M2抗原丰富.
- SSc-AM2A 透到活生生的小鼠肠道神经元和 HepG2 细胞,局部化到线粒体,并改变了细胞呼吸.
结论:
- 在SSc患者中,AM2A与消化道运动能力受损有关.
- 包括AM2A在内的SSc自身抗体可以透到活细胞并准线粒体,这表明一种致病机制.
- 通过影响富含线粒体的肠道神经元,AM2A可能会导致SSc中的肠道不运动.
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