基于高分辨率质谱法 (MALDI (+) -TOF MS和ESI(±) -Orbitrap MS) 的多组数据进行COVID-19的诊断查
Camila Medeiros de Almeida1, Larissa Campos Motta1, Gabriely Silveira Folli1
1Federal University of Espírito Santo- Chemistry Department, Vitória, ES, Brazil.
概括
血清脂质和蛋白质的多组学分析有效地区分了SARS-CoV-2感染. 像ESI-MS和MALDI-TOF MS这样的质谱技术为快速的临床诊断提供了高精度.
科学领域:
- 生物分子分析.
- 临床诊断 临床诊断 临床诊断
- 质谱测量质量谱测量
- 多种组合的多种组合.
背景情况:
- 随着COVID-19的流行,人们越来越需要快速诊断,而不仅仅是RT-PCR.
- 多组学分析提供了对COVID-19等疾病的更深入的生物学理解.
- 血清脂质和蛋白质组概况为感染提供了潜在的生物标志物.
研究的目的:
- 开发一种有效的多原子方法来区分SARS-CoV-2感染患者.
- 为诊断目的利用血清脂质和蛋白质组特征.
- 评估质谱在临床环境中的实用性.
主要方法:
- 使用电子喷雾电离质谱法 (ESI-MS) 和 Orbitrap 分析仪分析的脂质谱.
- 使用矩阵辅助激光溶解/离子化飞行时间质谱法 (MALDI-TOF MS) 分析的蛋白质组特征.
- 包括PCA,PLS-DA和SVM在内的统计分析被用于样本分类.
主要成果:
- 使用SVM模型,脂管学分析实现了高灵敏度 (高达100%) 和特异性 (高达96.88%).
- 使用MALDI-TOF MS和PLS-DA进行的蛋白质组学分析显示,高精度为99.10%.
- 这两种技术在区分SARS-CoV-2阳性和阴性样本方面都表现出强的表现.
结论:
- 与化学测量相结合的ESI-Orbitrap MS和MALDI-TOF MS对高灵敏度和特异性诊断非常有希望.
- 通过直接输注ESI-MS进行血清脂质分析是一种有效的临床诊断方法.
- 质谱学为临床应用提供了一种有价值,高吞吐量和最小侵入性的工具.
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