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Updated: Jun 13, 2026

Glutamine Flux Imaging Using Genetically Encoded Sensors
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Glutamine Flux Imaging Using Genetically Encoded Sensors

Published on: July 31, 2014

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使用光生物传感器测量哺乳动物细胞中D-氨基酸运输的协议

Danisha Johal1, Keeley G Hewton1, Sadie J Gale1

  • 1Department of Biochemistry & Molecular Biology, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.

STAR protocols
|June 29, 2025
PubMed
概括

这项研究引入了一种新方法,使用哺乳动物细胞中的基于酶的生物传感器实时可视化氨基酸运输. 这种技术有助于通过跟踪氨基酸运动来了解细胞代谢和疾病进展.

关键词:
癌症 癌症 癌症 癌症细胞膜的细胞膜.代谢过程中的代谢.分子/化学探头 分子/化学探头

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...