从大麻提取物中分离四大麻,使用离心分离色谱
Ada C Gallo-Molina1, César A Sánchez-Correa2, Iván D Gil-Chaves1
1Grupo de Procesos Químicos y Bioquímicos, Department of Chemical and Environmental Engineering, Universidad Nacional de Colombia - Sede Bogotá, Carrera 45 No 26-85, Bogotá, Colombia; Centro de Excelencia en Cannabis y Agronegocios, Universidad Nacional de Colombia, Colombia.
Journal of chromatography. A
|June 29, 2025
概括
这项研究开发了一种方法,使用,乙醇和水从大麻提取物中分离四大麻 (THC),从而产生符合监管标准的无THC宽谱产品.
科学领域:
- 化学工程是化学工程的重要组成部分.
- 制药科学 制药科学
- 自然产品化学 自然产品化学
背景情况:
- 监管要求要求有效地从大麻提取物中分离四大麻 (THC).
- 开发大规模,具有成本效益的净化方法对于大麻制品的制药和商业化至关重要.
- 溶剂选择至关重要,特别是考虑到制药限制和区域法规,影响工艺的可行性.
研究的目的:
- 开发和优化一种将THC与大麻提取物分离的方法,以生产一种无THC的产品.
- 鉴定适合大规模THC分离的溶剂系统,考虑到哥伦比亚的监管和制药约束.
- 评估该方法在生产广谱 (BS) 提取物和分离有价值的大麻素分离物的有效性.
主要方法:
- 使用ASPEN Plus V14进行过程模拟,以生成三元相图并确定最佳溶剂系统 (赫 / 乙醇 / 水).
- 使用吉尔森CPC 250进行实验室规模的测试,并使用Rothacrom rCPC开发工业规模的方法.
- 基于THC分割系数 (KTHC) 的溶剂系统的评估和使用全谱 (FS) 大麻提取物的性能测试.
主要成果:
- 一个七/乙醇/水系统 (4.6/3.5/1 v/v/v) 被选择用于工业规模的方法开发.
- 该过程成功地在9.5小时内从800克FS提取物中获得了613g的无THC广谱 (BS) 提取物 (85%CBD,0.02%THC) (77%的操作效率).
- 在残留分数和THC丰富分数 (85.3%THC) 的隔离中获得了高回收的cannabichromene (CBC) (97.06%).
结论:
- 开发的方法有效地将THC与大麻提取物分离,生产符合标准的无THC产品,适合大规模生产.
- 该过程允许在残留分数中丰富微量大麻素,如CBC,并将THC分离出来,用于潜在的医学研究.
- 这种方法提供了一个可行的解决方案,以满足监管要求,同时最大限度地提高大麻生物质的价值.
相关概念视频
01:11High-Performance Liquid Chromatography: Introduction
2.3K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
2.3K
01:05Centrifugation
2.6K
Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
2.6K
01:30Capillary Electrophoresis: Applications
540
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
540
01:05High-Performance Liquid Chromatography: Elution Process
694
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
694
01:10Chromatography: Introduction
4.7K
Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
4.7K
01:08Size-Exclusion Chromatography
792
In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
Silica particles offer advantages such as rigidity,...
792