在沙门氏菌感染期间,RNA结合蛋白 hnRNP A/B 控制了外体 miR-27a-5p 负荷
Mingjuan Qu1, Jianlong Zhang2, Xin Yu3
1School of Life Sciences, Ludong University, Yantai 264025, PR China; Collaborative Innovation Center for the Pet Infectious Diseases and Public Health in the Middle and Lower Stream Regions of the Yellow River, Yantai 264025, PR China; Shandong Engineering Research Center for Aquaculture Environment Control, Yantai 264025, PR China; Yantai Key Laboratory of Animal Pathogenic Microorganisms and Immunology, Yantai 264025, PR China.
Poultry science
|June 29, 2025
概括
沙门氏菌感染会改变巨细胞外体,重新编程它们的蛋白质和miRNA含量. 发现异质核核核糖核蛋白A/B (hnRNP A/B) 能够调节miR-27a-5p的外体输出,从而成为潜在的治疗标.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 沙门氏菌感染严重影响家禽和牲畜,造成经济损失.
- 巨细胞衍生的外体在沙门氏菌感染期间调节宿主炎症反应中发挥作用.
- 在炎症期间选择性miRNA包装到外体的机制尚不清楚.
研究的目的:
- 为了研究由沙门氏菌感染的巨细胞在外体中的蛋白质和miRNA变化.
- 为了识别参与miRNAs选择性包装成外体的蛋白质.
- 探索沙门氏菌病原性潜在的治疗点.
主要方法:
- 来自受感染和未受感染的巨细胞的外体的基于Tandem Mass Tag (TMT) 的定量蛋白质组学.
- RNA免疫沉 (RIP) 测试以确认蛋白质-miRNA相互作用.
- 异质核核核糖核蛋白A/B (hnRNP A/B) 的淘汰 (KO),以评估其在外体出口中的作用.
主要成果:
- 沙门氏菌感染显著重塑了外体蛋白质载荷,感染后12小时383种蛋白质上调和666种蛋白质下调.
- 基因和基因组的京都百科全书 (KEGG) 和基因本体学 (GO) 分析将目标基因与细菌传染病和分子结合联系起来.
- 异质核核核糖核蛋白A/B (hnRNP A/B) 在外体中被上调,并被证明可以结合miR-27a-5p,调节其外体出口.
结论:
- 在沙门氏菌感染期间, hnRNP A/B 是 miR-27a-5p 分类为外体的关键调节者.
- 调节hNRNP A/B功能可能会干扰沙门氏菌的发病和宿主免疫逃避.
- 这项研究确定了在兽医中控制沙门氏菌的潜在治疗目标.
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