由表皮和皮肤细胞组成的器官圈,以评估微塑料诱导的皮肤毒性
Yunxia Hu1, George Michael Nicolas2, Yongde Cai3
1Institute of Biopharmaceutical and Health Engineering (iBHE), Tsinghua Shenzhen International Graduate School (SIGS), Tsinghua University, Shenzhen, 518055, China; Key Lab of Industrial Biocatalysis, Ministry of Education, Shenzhen, 518055, China; Key Lab of Active Proteins and Peptides Green Biomanufacturing of Guangdong Higher Education Institutes, Tsinghua Shenzhen International Graduate School, Shenzhen, 518055, China.
Biomaterials
|June 29, 2025
概括
新的生物打印皮肤器官圈为测试微塑料 (MP) 毒性提供了一个有希望的替代方案. 这些模型显示了大小依赖的MP吸收,并揭示了UVA辐射的MP诱导显著的氧化应激和亡.
科学领域:
- 生物医学工程 生物医学工程
- 毒理学 毒理学 毒理学
- 材料科学 材料科学 材料科学
背景情况:
- 微塑料 (MP) 在消费品和环境中越来越普遍,对人类健康构成风险.
- 目前评估MP细胞毒性的方法有限,需要新的实验方法.
- 皮肤器官圈为评估MP对人类皮肤的影响提供了一个潜在的体外模型.
研究的目的:
- 开发和验证微流体珠喷印皮肤器官球,用于微塑料毒性评估.
- 为了研究微塑料对皮肤细胞的尺寸依赖吸收和影响.
- 评估紫外线辐射的微塑料对氧化应激和亡的影响.
主要方法:
- 从E18小鼠胎儿的表皮和皮肤细胞 (Epi-Dc) 生物打印到器官圈,使用微流体珠喷射技术.
- 具有不同尺寸的微塑料 (100 nm和500 nm) 的有机球的共同培养.
- 评估微塑料的吸收,细胞分化,氧化应激标志物 (ROS),亡和基因表达 (SOD,p53,Bax).
- 评估维生素C的保护作用和常规药物的细胞毒性.
主要成果:
- 生物打印的Epi-Dc器官圈表现出快速的自我聚合,迁移和分化.
- 观察到尺寸依赖的微塑料吸收,较小的MP显示更高的内部化.
- 100纳米的MP穿透了表皮层,而500纳米的MP仍然是外围的.
- 紫外线照射的MP显著增加了氧化应激,亡和ROS水平.
- 维生素C减轻了ROS水平,药物测定显示了剂量和时间依赖的细胞毒性.
结论:
- 埃皮-Dc器官圈是一种可行的高通量选系统,用于评估微塑料的皮肤毒性.
- 该模型有效地证明了尺寸依赖的微塑料吸收和紫外线诱导的损害.
- 这种生物打印方法促进了与皮肤相关的应用程序的快速药物测试和毒理评估.
相关概念视频
01:16Sensory Functions of the Skin
The skin is the largest organ of the human body and plays a crucial role in our sensory perception. It contains a vast network of sensory receptors that contribute to the skin's protective function by perceiving physical, biological, and environmental cues and generating relevant responses.
There are two main categories of receptors on the skin: capsulated and non-capsulated. The non-capsulated ones are mainly the pain receptors. The capsulated ones can be further categorized based on the...
There are two main categories of receptors on the skin: capsulated and non-capsulated. The non-capsulated ones are mainly the pain receptors. The capsulated ones can be further categorized based on the...
01:11Papillary Dermis
Dermis
The dermis might be considered the "core" of the integumentary system, as distinct from the epidermis and hypodermis. It contains blood and lymph vessels, nerves, and other structures, such as hair follicles and sweat glands. The dermis is made of two layers of connective tissue that comprise an interconnected mesh of elastin and collagenous fibers, produced by fibroblasts.
Papillary Layer
The papillary layer is made of loose, areolar connective tissue, which means the collagen and...
The dermis might be considered the "core" of the integumentary system, as distinct from the epidermis and hypodermis. It contains blood and lymph vessels, nerves, and other structures, such as hair follicles and sweat glands. The dermis is made of two layers of connective tissue that comprise an interconnected mesh of elastin and collagenous fibers, produced by fibroblasts.
Papillary Layer
The papillary layer is made of loose, areolar connective tissue, which means the collagen and...