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Ctenopharyngodon idellus Mex3C通过促进RIG-I的K63连接的无处可见性来激活天生的免疫系统

Tingting Yu¹, Chuxin Wu², Dongming Li³

¹State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang, 330047, Jiangxi, China; Department of Bioscience, College of Life Science, Nanchang University, Nanchang, 330031, Jiangxi, China.

Fish & shellfish immunology

|June 29, 2025

概括

草Mex3C (CiMex3C) 作为E3泛素结合酶,增强RIG-I泛化并提高抗病毒先天免疫力. 这一发现突出了CiMex3CC的发现.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

细胞质RNA传感器,RIG-I类受体 (RLRs),对于抗病毒先天免疫是至关重要的.
草的Mex3C基因 (CiMex3C) 编码了一个具有RING和KH域的蛋白质.

研究的目的:

为了研究草CiMex3C在抗病毒天生的免疫力中的作用.
为了确定CiMex3C是否调节RIG-I激活.

主要方法:

在草鱼中识别和描述CiMex3C.
在CIK细胞中对CiMex3C和RIG-I的同局部化研究.
评估CiMex3C的E3泛基因酶活性及其对RIG-I泛基因化和IFN I产生的影响.

主要成果:

CiMex3C表达被病毒刺激 (GCRV,多I:C),B-DNA,Z-DNA) 上调,对GCRV的敏感性增加.
在细胞质中,CiMex3C与RIG-I同定位,并增强了RIG-I K63链接的无处不在.
CiMex3C显示了E3泛素酶活性,直接催化了RIG-I的K63相关的泛化.
抑制CiMex3C减少了RIG-I无化,并抑制了IFN I的产生.

结论:

CiMex3C作为RIG-I激活的翻译后调制器.
CiMex3C在远视动物的抗病毒天生的免疫反应中起着至关重要的作用.

关键词:

国际货币基金组织 (IFN) I 这是天生的免疫力.墨西哥墨西哥墨西哥在RIG-I中,RIG-I是指RIG-I.在Ubiquitination中使用.

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