炎症期间的脂质代谢适应受昼夜时钟的控制,并在夜间受到光线的影响
Beata Benedikova1, Viera Sebenova Jerigova1, Michal Zeman1
1Department of Animal Physiology and Ethology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
概括
昼夜时钟调节了对炎症的代谢反应,夜间人工光的干扰会损害脂质代谢. 这凸显了时钟在免疫防御和代谢适应中的作用.
科学领域:
- 免疫学 免疫学 免疫学
- 代谢过程中的代谢.
- 时间生物学 时间生物学
背景情况:
- 免疫防御需要全身代谢转变来支持激活的免疫细胞.
- 昼夜时钟影响免疫反应,但其在免疫挑战后代谢适应中的作用尚不清楚.
- 夜间的人工光 (ALAN) 破坏了自然节奏,可能会影响免疫和代谢过程.
研究的目的:
- 在内毒素刺激后,研究大鼠肝脏和内脏白脂肪组织 (vWAT) 的日间依赖性炎症和代谢反应.
- 为了检查ALAN如何影响这些反应并破坏周围代谢时钟.
- 为了阐明昼夜调节和在炎症期间的代谢适应之间的联系.
主要方法:
- 在正常的光/黑暗周期或ALAN下,雄性大鼠在白天或夜晚接受脂聚糖化物 (LPS) 的挑战.
- 肝脏和vWAT中的代谢途径和外周时钟在系统和分子层面上进行了分析.
- 评估了炎症标志物,包括NFIL3,NF-κB和NLRP3炎症体.
主要成果:
- 在正常光照下,LPS注射诱导了依赖于一天时间的脂肪酸 (FA) 调动和vWAT和肝脏中的葡萄糖代谢.
- 炎症途径 (NFIL3,NF-κB/NLRP3) 与这些日常代谢变化相关.
- 阿兰在炎症期间扰乱了代谢时钟和受损的脂质代谢适应.
结论:
- 循环时钟在LPS诱导的炎症期间,在调节葡萄糖和FA代谢方面发挥着至关重要的作用.
- 夜间的人工光显著扰乱了这些循环节控制的代谢适应.
- 了解这些相互作用对于在光线中断环境中管理免疫和代谢健康至关重要.
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