评价六种商业和非商业素抗性诊断的评估
Tumisho Mmatumelo Seipei Leshaba1, Masego Mmatli1, Nontombi Marylucy Mbelle1
1Department of Medical Microbiology, School of Medicine, University of Pretoria, Pretoria, South Africa.
MicrobiologyOpen
|June 30, 2025
概括
全球范围内,素耐药性正在上升. 维特克2和Sensititre为胆固醇最小抑制度 (MIC) 测试提供了最佳性能,而Rapid NP则提供了快速选.
科学领域:
- 临床微生物学 临床微生物学
- 抗微生物耐药性 抗微生物耐药性
- 诊断试验评价 诊断试验评价
背景情况:
- 胆固醇是一种关键的最后手段抗生素,用于治疗多药耐药的格兰氏阴性感染.
- 全球抗素耐药性日益增加,需要准确高效的诊断方法.
- 有几种商业和内部诊断测试可用于检测胆固醇抗性.
研究的目的:
- 评价和比较六种胆固醇抗性诊断的性能与黄金标准.
- 为了确定每个测试的灵敏度,特异性,分类一致性和错误率.
- 引导临床实验室选择合适的诊断工具.
主要方法:
- 根据ISO标准使用汁微稀释 (BMD) 作为参考方法.
- 测试了142种格兰阴性分离物 (Enterobacterales,Acinetobacter baumannii,Pseudomonas aeruginosa) 的研究结果.
- 对mcr基因 (mcr-1至mcr-5) 进行了多重PCR查.
主要成果:
- 维特克2和Sensititre显示了最高的分类一致性 (≥98%) 和没有非常大的错误.
- 康马斯普对Acinetobacter baumannii表现出色,但对Pseudomonas aeruginosa表现较差.
- CHROMagar COL-APSE在肠杆菌中具有低特异性 (69%) 和高重大错误;快速NP测试显示了很高的非常重大错误率 (7.84%).
结论:
- 维特克2和Sensititre是对胆固醇MIC测试的最佳选择,ComASP作为一个具有成本效益的替代方案.
- 快速NP测试提供快速选,但需要确认MIC测试.
- CHROMagar COL-APSE需要仔细的注射控制,以尽量减少假抗体的检测.
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