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Updated: Sep 17, 2025

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

Published on: June 23, 2023

一个半自动体积相关光显微镜和传输电子断层扫描的工作流

Kohki Konishi^1,2,3, Guilherme Neves³, Matthew Russell⁴

¹Nikon U.K., Branch of Nikon Europe B.V., Surrey, UK.

Journal of microscopy

|June 30, 2025

概括

我们开发了SegReg和NavROI工具,以改善体积相关光和电子显微镜 (vCLEM) 的导航. 这些工具提高了分子定义的亚细胞域的超结构分析的效率.

科学领域:

细胞生物学细胞生物学
显微镜技术显微镜技术
神经科学是一个神经科学.

背景情况:

卷相关光和电子显微镜 (vCLEM) 能够对特定细胞区域进行超结构分析.
在多模式和多尺度vCLEM数据集中有效导航感兴趣的区域 (ROI) 仍然是一个重大挑战.

研究的目的:

在vCLEM中开发和整合有效导航和ROI定位的工具.
为了提高超结构性研究的vCLEM工作流程的可访问性和效率.

主要方法:

开发 SegReg,一个使用细分的GPU加速多模式图像注册工具.
开发NavROI,3D图像查看器,用于可视化多式模式图像注册.
集成应用SegReg和NavROI用于导航小鼠大脑组织块.

主要成果:

证明了大型组织块的成功导航,并保留了光.
实现了ROI的TEM断层扫描的精确准,包括突触和轴突细胞器官.
提供切割,切割深度估计和视觉导航的实时指导.

结论:

集成的SegReg和NavROI工作流显著提高了vCLEM分析的效率和可访问性.
这种方法促进了对特定亚细胞领域的详细超结构性研究,例如神经元突触和细胞器官.

关键词:

自动化管道自动化管道相关光和电子显微镜相关光和电子显微镜.电子断层扫描是一种电子断层扫描.图像处理是图像处理的过程.显微镜样本导航导航突触超结构的突触超结构.

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