在不同的测试条件下,使用循环瘤DNA预测结肠直肠癌复发的14个基因小组
Yuichi Hisamatsu1, Koji Ando1, Kensuke Kudo1
1Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Cancer science
|June 30, 2025
概括
在手术后检测结直肠癌患者的最小残留疾病 (MRD) 是至关重要的. 一种无瘤循环瘤DNA测定显示出检测MRD和预测复发的前景,提供了一种具有成本效益的临床方法.
科学领域:
- 在瘤学瘤学.
- 分子诊断学 分子诊断
- 遗传学 遗传学 是一个
背景情况:
- 在结直肠癌手术后精确检测最小残留疾病 (MRD) 对于预测复发至关重要.
- 传统的方法,如组织学和CEA检测缺乏足够的灵敏度来检测MRD.
- 循环瘤DNA (ctDNA) 是对结直肠癌MRD评估的新兴生物标志物.
研究的目的:
- 评估针对可切除结直肠癌中ctDNA检测的14基因面板试验 (Sysmex Plasma-Safe-SeqS) 的有效性.
- 为了比较瘤信息,血信息和瘤原始ctDNA测定在检测MRD的性能.
- 评估术后ctDNA对结直肠癌复发的预测价值.
主要方法:
- 招募了46名患有II期结直肠癌的日本患者.
- 血样本在手术前和手术后采集用于ctDNA分析,使用Sysmex Plasma-Safe-SeqS试验.
- 使用了三种测试方法:瘤知情,血知情和瘤无知. 患者的随访时间平均为1169天.
主要成果:
- 在98%的病例中检测到瘤变体.
- 在70%的患者中检测到术前的ctDNA,在35%的患者中检测到术后的ctDNA.
- 瘤原始试验发现了更多的手术后ctDNA阳性病例,并显示了预测复发的潜力.
结论:
- 瘤原始ctDNA测定表明了在结直肠癌中检测MRD的潜力.
- 这种方法在日常临床实践中提供了成本和实施方面的优势.
- 需要进行进一步的大规模研究,以优化对结直肠癌复发的ctDNA检测策略.
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