相关实验视频

Updated: Sep 17, 2025

Chemogenetic Regulation in Reprogrammed Stem Cell-derived Precursor Cells in Treating Neurodegenerative Diseases

Chemogenetic Regulation in Reprogrammed Stem Cell-derived Precursor Cells in Treating Neurodegenerative Diseases

Published on: May 2, 2025

大麻二醇及其主要代谢物的对人类神经干细胞的影响

Leah E Latham¹, Qiang Gu¹, Shuliang Liu¹

¹Division of Neurotoxicology, National Center for Toxicological Research/Food and Drug Administration, Jefferson, AR, United States.

Experimental biology and medicine (Maywood, N.J.)

|June 30, 2025

概括

大麻素 (CBD) 和其代谢物损害人类神经干细胞的活力,并影响胎儿大脑发育. 暴露于CBD,7-OH-CBD和7-COOH-CBD会影响细胞生长和受体表达,表明潜在的神经发育风险.

科学领域:

神经科学是一个神经科学.
发展生物学发展生物学
药理学药理学是指药理学的学科.

背景情况:

Cannabidiol (CBD) 的使用正在增加,怀孕期间有可能发生胎盘转移.
有关胎儿暴露于CBD及其代谢物 (7-OH-CBD,7-COOH-CBD) 影响神经发育的担忧.
德尔塔-9四大麻素 (THC) 常用于大麻素研究中的比较.

研究的目的:

研究CBD及其主要代谢物对人类神经干细胞 (NSC) 的影响.
评估CBD及其代谢物对胎儿大脑发育标记物的影响.
为了比较CBD和THC对NSC活力,增殖和分化的影响.

主要方法:

人类神经干细胞 (NSC) 暴露于CBD,7-OH-CBD和7-COOH-CBD,其度和持续时间各不相同.
为了进行比较分析,NSC也被用delta-9四大麻 (THC) 治疗.
在治疗后分析了细胞活力,增殖,分化标志物 (GFAP,CB1,CB2,β-素III).

主要成果:

CBD,7-OH-CBD和7-COOH-CBD以剂量依赖的方式降低了NSC的活力.
在G1阶段,CBD和7-OH-CBD降低了NSC数量;在相关度的更长时间暴露会增加细胞死亡.
在分化后,CBD减少了GFAP和CB2的表达;THC减少了GFAP的表达,突出了神经发育的脆弱性.

结论:

临床相关的CBD,7-OH-CBD和7-COOH-CBD度对人类NSC生理学产生不利影响.
在分化细胞中CB2受体和GFAP的减少表达表明中枢神经系统在发育过程中对CBD和THC的脆弱性.
这些发现为体内神经发育研究提供了背景,强调了人类代谢物概况.

关键词:

7-COOH-CBD是什么意思 7-COOH-CBD是什么意思 7-OH-CBD的使用方法这就是为什么CBD是CBDCBD是CBD.在这里,我们可以看到THCTHCTH.神经干细胞的神经干细胞

更多相关视频

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases

Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases

Published on: June 28, 2019

相关概念视频

CNS Stimulants: Cocaine, Amphetamines and Cannabinoids

CNS Stimulants: Cocaine, Amphetamines and Cannabinoids

CNS stimulants, such as cocaine, amphetamines, and cannabinoids, have varying structures and mechanisms of action that lead to different therapeutic effects and side effects. Cocaine, with its molecular formula C17H21NO4, is a tropane alkaloid and a tertiary amino compound. It has two chemical forms: the hydrochloride salt and the "freebase." The former is in powder form, while the latter involves removing the hydrochloride salt to create a form that can be smoked. Cocaine exerts its...