Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Sequence-Dependent Folding of Recognition-Encoded Melamine Oligomers.

Journal of the American Chemical Society·2026
Same author

Profiling Protein Aggregate Size Using Single-Molecule Array Technology.

Analytical chemistry·2026
Same author

PD-1 signaling and PD-1 blockade-mediated tumor control are established at microvillar T cell contacts.

Science immunology·2026
Same author

Development of novel chalcone-based quinazoline derivatives as dual VEGFR-2 and EGFR inhibitors.

RSC medicinal chemistry·2026
Same author

Single-molecule detection methods to study alpha-synuclein aggregation in postmortem Parkinson's disease brains.

Cell reports methods·2026
Same author

A Correlative SICM-OPM Platform for Surface and Volumetric Imaging in Live Cells.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

Tunable redox hopping charge transport and electrochromism in multivariate MOFs: effects of substitution patterns and number of sulfonic acid groups.

Chemical science·2026
Same journal

Dearomative [2 + 2] photocycloaddition to difluoro bicyclo[2.1.1]hexane bioisosteres.

Chemical science·2026
Same journal

Chemoproteomics unveils the antibacterial mechanism of silver ions: inhibiting peptidoglycan synthesis <i>via</i> targeting Mur family proteins in <i>Staphylococcus aureus</i>.

Chemical science·2026
Same journal

PFAS free chemically amplified resists enabled by low activation energy hydrocarbon cage monomers.

Chemical science·2026
Same journal

Membrane anchoring amplifies intracellular catalysis of caged palladium nanoclusters.

Chemical science·2026
Same journal

Bistelluridopnictanes as gateways to telluradipnictiranes and a cyclic [Bi<sub>2</sub>Te<sub>2</sub>]<sup>2+</sup> dication.

Chemical science·2026
查看所有相关文章

相关实验视频

Updated: Sep 17, 2025

Detection of Disease-associated &#945;-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated &#945;-synuclein
12:01

Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein

Published on: May 30, 2015

10.8K

在单分子阵列上检测具有小分子的α-synuclein聚合物.

Jeff Y L Lam1,2,3, Timothy S Chisholm1, Hadia Almahli1

  • 1Yusuf Hamied Department of Chemistry, University of Cambridge Cambridge CB2 1EW UK mrc79@cam.ac.uk ch664@cam.ac.uk dk10012@cam.ac.uk.

Chemical science
|June 30, 2025
PubMed
概括
此摘要是机器生成的。

化学合成的小分子现在可以使用单分子阵列 (SiMoA) 试验检测阿尔法-同核素聚合物,这是帕金森病的关键生物标志物. 这项创新为早期神经退行性疾病诊断提供了一种多功能和高效的方法.

更多相关视频

Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils Formation
09:16

Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils Formation

Published on: June 26, 2018

7.7K
Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains
09:27

Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains

Published on: January 5, 2016

17.5K

相关实验视频

Last Updated: Sep 17, 2025

Detection of Disease-associated &#945;-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated &#945;-synuclein
12:01

Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein

Published on: May 30, 2015

10.8K
Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils Formation
09:16

Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils Formation

Published on: June 26, 2018

7.7K
Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains
09:27

Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains

Published on: January 5, 2016

17.5K

科学领域:

  • 生物化学 生物化学
  • 神经科学是一个神经科学.
  • 分析化学 分析化学

背景情况:

  • 蛋白质聚合物,如α-synuclein,是早期诊断神经退行性疾病的关键生物标志物,如帕金森病.
  • 单分子阵列 (SiMoA) 平台使生物流体中生物标志物的超低度检测成为可能.
  • 目前的SiMoA测试通常依赖于抗体来捕获向分子,其开发可能是劳动密集的.

研究的目的:

  • 开发下一代SiMoA试验,利用化学合成的小分子作为α-synuclein聚合物的捕获剂.
  • 与传统的基于抗体的方法相比,评估基于小分子的结合剂的疗效和特异性.
  • 增强SiMoA平台的多功能性和适应性,用于生物标志物发现和诊断.

主要方法:

  • 设计用于结合α-synuclein聚合物的小分子的化学合成.
  • 小分子与初级氨基的功能化,以与SiMoA珠子结合.
  • 使用复合和人类血液样本测试合成分子 (例如BF-79-2) 的捕获效率和特异性.

主要成果:

  • 一种新型的小分子BF-79-2,证明了对α-synuclein聚合物的皮科莫尔捕获.
  • BF-79-2分子特别捕获聚合物,但不包括单体.
  • 在使用基于小分子的SiMoA测定方法在人类血液样本中成功检测了α-synuclein聚合物.

结论:

  • 小分子可以有效地替代抗体作为捕获剂,用于检测蛋白质聚合物的SiMoA试验.
  • 这种方法提高了测试的多功能性,使得选和简化合成成为可能.
  • 这项开发扩大了SiMoA平台的功能,用于生物标志物发现和早期诊断同核蛋白病变.