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PpSKα通过激活桃子果中的PpTrxh9来提高冷却耐受性

Caifeng Jiao¹, Jing Sun²

¹School of Horticulture, Anhui Agricultural University, Hefei, China.

Frontiers in plant science

|June 30, 2025

概括

甘氨酸贝他因通过提高SHAGGY相关蛋白激酶a (PpSKa) 表达来提高桃子的寒冷耐受性. 这种激酶酸化硫素h9 (PpTrxh9),减少果实中的冷却损伤和氧化损伤.

科学领域:

植物生理学植物生理学
生物化学生物化学
收获后的生物学

背景情况:

冷却损伤 (CI) 显著降低收获后的水果质量,特别是桃子,影响市场价值.
冷链系统虽然对保存至关重要,但可以在易受影响的水果中诱导CI.

研究的目的:

调查糖氨酸贝他因 (GB) 在减轻桃子果伤害中的作用.
阐明GB诱导的寒冷耐受性背后的分子机制,重点关注蛋白质相互作用和基因表达.

主要方法:

酵母两杂交,共免疫沉,拉下,和双分子光补充测试以确定蛋白质相互作用.
在桃子和番茄水果中进行基因表达分析 (过度表达和病毒诱导的基因沉默).
激酶测试以确定酸化活性.

主要成果:

补充糖氨酸贝他因 (GB) 增强了桃子的寒冷耐受性,并通过上调 thioredoxin h9 (PpTrxh9) 表达,减少了桃子的氧化损伤.
与SHAGGY相关的蛋白激酶a (PpSKa) 被确定为与PpTrxh9相互作用的蛋白质,其表达因GB治疗而升高.
过度表达PpSKa降低了番茄果的寒冷敏感性,而在桃子果的沉默加剧了CI和氧化损伤.
证实PpSKa可酸化PpTrxh9,这表明它具有直接的调节作用.

结论:

与SHAGGY相关的蛋白激酶a (PpSKa) 在降低桃子水果中降温损伤的发生方面发挥着至关重要的作用.
PpSKa通过增强硫素h9 (PpTrxh9) 的表达和/或活性来减轻CI,从而改善耐寒性并减少氧化损伤.

关键词:

与SHAGGY相关的蛋白质激酶αα.制冷耐受度制冷耐受度氧化损伤是因为氧化损伤.桃子果实是一种桃子果实.甲素H9的使用方法

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