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在除压力疾病中,通过热冲击因子1对内皮细胞损伤进行高压氧预处理

Caiyi Xu¹, Quan Zhou¹, Xiangyang Meng¹

¹Naval Medical Center, Naval Medical University, Shanghai, China.

Frontiers in molecular biosciences

|June 30, 2025

概括

高压氧预处理通过激活ROS/AKT/HSF-1通路,增加热冲击蛋白质,防止气泡损伤和减压疾病. 这增强了血管内皮细胞的抵抗力,并减轻了老鼠的损伤.

科学领域:

生物医学工程生物医学工程
细胞生物学细胞生物学
生理学生理学生理学

背景情况:

解压性疾病 (DCS) 存在由于气泡诱导的血管内皮细胞 (VEC) 损伤的风险.
高压氧 (HBO) 预处理正在研究其对VEC损伤和DCS的保护作用.
热冲击因子1 (HSF-1) 在HBO的保护机制中的作用是一个关键的焦点.

研究的目的:

阐明HBO预处理对泡诱导的VEC损伤的保护机制.
调查热冲击因子1 (HSF-1) 信号通路在HBO的保护作用中的参与.
在大鼠模型中评估HBO预治疗在预防减压疾病 (DCS) 的有效性.

主要方法:

使用初级培养的老鼠肺微血管内皮细胞 (PMVECs) 和斯普拉格-道利老鼠.
通过HBO暴露后的西方斑点分析检查HSF-1激活和下游蛋白质表达.
采用特定的抑制剂和siRNAs来划分信号通路,并评估在防治泡引起的伤害和DCS中的功能性作用.

主要成果:

在ROS/AKT/HSF-1通路的介导下,HBO预处理在PMVEC和老鼠肺部上调了HSF-1,HSP27和HSP40的表达.
在细胞泡损伤模型中,HBO预治疗显著降低了VEC活力丧失,内皮微粒和炎症标记物.
在大鼠DCS模型中,HBO预治疗降低了肺损伤标志物和炎症,HSF-1抑制逆转了益处.

结论:

通过ROS/AKT/HSF-1通路,HBO预处理可以增强VEC对泡损伤的抵抗力.
HBO对HSP27和HSP40的升级有助于减轻与DCS相关的损害.
HSF-1信号通路对于HBO对气泡诱导损伤和DCS的保护作用至关重要.

关键词:

压力下降疾病是压力下降疾病.热冲击因子 1 1 的热冲击因子高压氧气的高压氧气.保护性蛋白质是一种保护性蛋白质.肺部的微血管内皮细胞.

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