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鉴定,优化和生产的xylooligosaccharides通过耐热的氨酶产生菌株

Xueyu Qiu^1,2, Qijie Sun², Chunyan Huo²

¹Key Laboratory of Geriatric Nutrition and Health Ministry of Education, Beijing Technology and Business University, No. 11 Fucheng Street, Haidian District, Beijing, 100084 China.

|June 30, 2025

概括

一种新型的耐热西兰酶产生菌株,Thermomyces dupontii J22,被优化为高细胞质无菌酶生产. 这种酶能有效地从玉米中产生氧化氧化糖 (XOS).

科学领域:

生物技术是生物技术.
酶学是一种酶学.
微生物发酵微生物发酵

背景情况:

克西兰酶酶对于分解植物细胞壁的主要组成部分 - - 克西兰而言至关重要.
在工业应用中,开发强大的生产紫外线酶的微生物菌株是必不可少的.
玉米是一种丰富的基纤维素生物质,适合酶化水解.

研究的目的:

为了隔离和描述一种新型耐热酶生成菌株.
为了优化发酵条件,最大限度地生产西兰酶.
为了评估产生的酶在产生氧化氧化糖 (XOS) 的有效性.

主要方法:

隔离和鉴定一种耐热微生物菌株 (Thermomyces dupontii J22).
优化液体发酵参数,包括碳/来源,pH和温度,使用单因子和响应表面方法.
玉米水热提取物的酶化水解,使用原始西兰酶酶产生XOS.

主要成果:

在优化的条件下,Thermomyces dupontii J22产生了高水平的无细胞酶西兰酶 (768.32 U/mL) (5%的玉米,3%的源 (3:1:1酵母糊:三:氨二酸),pH7.2,47.5°C).
原始西兰酶在80°C和pH值为6时表现出最佳活性.
从玉米提取物中酶性生产XOS,在60°C和pH值7.0时,产生了富含西洛比和西洛的产品 (74.29%的总成分).

结论:

热菌 (Thermomyces dupontii J22) 是一个有前途的耐热西兰酶来源,可用于工业应用.
优化的发酵条件显著提高了酶产量.
产生的西兰酶是有效的酶合成有价值的西洛酸糖从林氏纤维素生物质.

关键词:

优化优化优化优化这种植物是Thermomyces dupontii.克西兰酶的生产.氧化氧糖 (XOS) 是一种氧化酸盐.

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