增强从重组糖菌 (Saccharomyces cerevisiae) 提升3-基酸的生产,以使用米水解和甘工业废物作为基质
Supattra Lertsriwong1,2, Phatcharaphong Khaosaart1,2, Nassapat Boonvitthya3
1Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
Synthetic and systems biotechnology
|June 30, 2025
概括
研究人员通过改造马洛尼尔-CoA减酶 (MCR) 酶,改善了从纤维纤维素生物质中生产3-基酸 (3-HP) 的生产. 这一策略实现了10g/L的标位,为工业3HP合成提供了可持续的途径.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 可持续化学 可持续化学
背景情况:
- 纤维素生物质,如大米和甘废物,是可转化为微生物生产糖的多糖类的丰富来源.
- 3-基酸 (3-HP) 是一种有价值的生物基化学构件,具有重要的工业潜力.
- 马洛尼尔-CoA途径,利用马洛尼尔-CoA减少酶 (MCR) 酶,是3-HP生物合成的关键途径,但酶活性失衡限制了效率.
研究的目的:
- 通过解决双功能MCR酶中的活性失衡来增强3-HP的产生.
- 增加中间供应并优化马洛尼尔-CoA通路,以获得更高的3HP产量.
- 开发一种可持续的方法,用于在工业规模生产3HP的纤维素生物质.
主要方法:
- 将双功能MCR酶剖析成两个片段,以提高催化效率.
- 设计一种重组酵母菌株 (*Saccharomyces cerevisiae*),以表达修改后的*mcr*基因.
- 用烯和酸盐补充发酵介质,以提高中间供应和3HP产量.
- 使用水解米作为发酵的可再生碳来源.
主要成果:
- 与全长基因菌株相比,与切割的*mcr*基因重组菌株的3-HP标位增加了21倍.
- 添加烯酸和酸盐增加了3-HP产量8倍,达到10g/L (0.201g<子产品>/g<子基质>) 的标位.
- 使用稀释的水解米的发酵产生了4.02g/L的3-HP (0.074g<副产品>/g<副糖>).
结论:
- 剖析MCR酶有效平衡其活性,显著改善3HP产量.
- 优化的发酵条件和基质利用为可持续的3HP制造提供了可行的策略.
- 这项研究提出了一种有前途的方法,用于工业规模的价值化基纤维素废弃物到有价值的化学品.
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