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相关概念视频

RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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相关实验视频

Updated: May 6, 2026

Use of Alu Element Containing Minigenes to Analyze Circular RNAs
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Use of Alu Element Containing Minigenes to Analyze Circular RNAs

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CircPac:一个基于网络的分析工具集,用于探索循环RNA数据.

Amir Hossein Foroutan1, Sadra Salehi-Mazandarani2, Maryam Lotfi-Shahreza1,3

  • 1Department of Computer Engineering, Shahreza Campus, University of Isfahan, Isfahan, Iran.

Data in brief
|June 30, 2025
PubMed
概括

一个新的网络工具CircPac帮助研究人员分析循环RNA (circRNA) 数据. 它集成了多个数据库,可视化circRNA信息,miRNA目标和与疾病相关的表达变化.

关键词:
一个圆形的圆形圆形.计算生物学是一种计算生物学.数据可视化数据可视化微RNAs是一种微RNA.这是一个RNARNARNARNARNA.基于网络的分析分析.

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Identification of Circular RNAs using RNA Sequencing
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Identification of Circular RNAs using RNA Sequencing

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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

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相关实验视频

Last Updated: May 6, 2026

Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.4K
Identification of Circular RNAs using RNA Sequencing
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Identification of Circular RNAs using RNA Sequencing

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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

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科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 基因组学就是基因组学.

背景情况:

  • 循环RNAs (circRNAs) 是关键的调节分子,具有独特的特性,如组织特异性表达和稳定性.
  • 现有的分析工具缺乏用于circRNA数据分析的全面功能.
  • 需要集成的平台来探索circRNA功能和疾病关联.

研究的目的:

  • 开发和介绍CircPac,一个用户友好的,基于Web的工具集,用于全面的circRNA数据分析.
  • 整合来自主要circRNA数据库 (circBase,circBank,circRNADisease) 的数据.
  • 为了使circRNA信息,miRNA相互作用和疾病特异性的表达模式可视化.

主要方法:

  • 使用Python,pandas,seaborn和Django开发一个基于Web的工具集.
  • 集成多个circRNA数据库.
  • 实现circRNA ID统一,信息检索,miRNA目标识别和表达分析的功能.

主要成果:

  • CircPac为访问和分析circRNA数据提供了一个统一的平台.
  • 该工具集有助于识别circRNA-miRNA相互作用.
  • CircPac可以可视化各种疾病中的circRNA表达变化,生成准备发布的数据.

结论:

  • CircPac为生物学家提供了一个宝贵的资源,可以有效地分析和可视化circRNA数据.
  • 该工具集简化了与circRNAs相关的复杂生物信息分析.
  • CircPac支持研究circRNAs在生物过程和疾病中的作用.