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基于BgaR的乳糖生物传感器的构建和优化,用于对人类牛奶寡糖生产者的高通量选择

Junzhong Li¹, Tingyu He¹, Shaodong Ren¹

¹Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, National Engineering Laboratory for Industrial Enzymes, Tianjin 300457, P. R. China.

Journal of agricultural and food chemistry

|June 30, 2025

概括

这项研究开发了一种新的生物传感器,通过优化乳糖生物传感器来检测人乳寡糖化物 (HMO). 这项创新使高通量选 (HTS) 有效,从而改善HMO生产.

科学领域:

生物技术是生物技术.
微生物学微生物学
代谢工程是代谢工程.

背景情况:

高通量查 (HTS) 对于识别代谢物过度生产者至关重要.
人类牛奶寡糖化物 (HMO) 生物传感器稀缺,限制了有效的查.
乳糖检测以前被用作HMO度的代理.

研究的目的:

开发和优化人类牛奶寡糖类 (HMO) 的通用生物传感器.
改进乳糖生物传感器的检测和动态范围,以提高灵敏度.
为HMO过度生产者提供高效的HTS.

主要方法:

开发了一种使用BgaR转录因子的乳糖生物传感器,将乳糖度与菌株生长联系起来.
优化了生物传感器,将检测范围从5-10 g/L扩大到0-12 g/L,动态范围扩大了5.19倍.
引入并微调 LacI 表达,由 BgaR 控制,以适应生物传感器用于 HMO 检测.

主要成果:

实现了乳糖生物传感器检测和动态范围的显著扩展.
通过HTS.成功获得了超产的Lacto-N-triose II和2'-fucosyllactose (2'-FL).
确定了一种新的T1124C突变,该突变可以将2'-FL的产生提高到78.21 ± 2.10 g/L.

结论:

这项研究提出了一种用于构建和优化通用HMO生物传感器的多功能方法.
开发的生物传感器为HMO过度生产者提供了高效的HTS.
这些发现有助于HMO生产和代谢工程的进步.

关键词:

生物传感器生物传感器增长合增长合高通量选的高通量选人类牛奶中的寡糖化物转录因子的转录因子

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