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Updated: Sep 17, 2025

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
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通过单颗粒追踪实时监测DNA原始细胞相互作用

Indra Van Zundert1, Elena Spezzani1, Roger R Brillas1

  • 1Biomedical Engineering Department, Technische Universiteit Eindhoven, Het Kranenveld 14, 5612 AZ, Eindhoven, The Netherlands.

Small (Weinheim an der Bergstrasse, Germany)
|June 30, 2025
PubMed
概括

用连接体功能化的DNA原始纳米棒有选择性地准癌细胞,揭示实时分子相互作用. 这项研究增强了对生物医学应用的细胞接口的DNA原形行为的理解.

关键词:
基因原始是指DNA的原始形式.细胞-纳米粒子相互作用配体-受体结合动态 配体-受体结合动态单颗粒追踪器是一个单颗粒追踪器.

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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