在高葡萄糖和炎症条件下,BFGF降低细胞内膜网膜应激的压力,以减轻HUVEC细胞损伤
Fei Hu1, Wei Shen1, Longyang Xu1
1Cixi Biomedical Research Institute, Wenzhou Medical University, Cixi, Ningbo, China.
Endokrynologia Polska
|June 30, 2025
概括
基本纤维细胞生长因子 (bFGF) 在高葡萄糖和炎症条件下改善人类静脉内皮细胞功能. 它增强了细胞活动,迁移和血管功能,同时减少了细胞亡和内质网膜应激 (ERS).
科学领域:
- 血管生物学 血管生物学
- 内分泌学 在内分泌学.
- 细胞和分子医学 细胞和分子医学
背景情况:
- 糖尿病血管病,糖尿病的并发症,是导致死亡的主要原因.
- 基本纤维细胞生长因子 (bFGF) 具有不同的生理作用.
- 对糖尿病血管损伤的bFGF的研究提供了潜在的新治疗策略.
研究的目的:
- 评估bFGF在糖尿病血管病变中的治疗潜力.
- 确定bFGF在高葡萄糖和炎症条件下对人类静脉内皮细胞 (HUVEC) 的影响.
- 阐明bFGF的作用背后的分子机制,重点关注细胞内膜网膜应激 (ERS).
主要方法:
- 培养了HUVEC细胞,并接受了高葡萄糖和炎症刺激.
- 细胞计数套件8 (CCK8) 试验评估了细胞活力和最佳bFGF/干扰素- (IFN-γ) 度.
- 斯克拉奇和特朗斯韦尔测定细胞迁移;西部斑 (WB) 和免疫光 (IF) 分析了蛋白质表达 (亡,增殖,血管功能,ERS).
- 聚合酶链反应 (PCR) 量化了ERS基因表达;管形成试验评估了血管生成.
主要成果:
- bFGF在体外显著增强了HUVEC细胞活动和迁移能力.
- bFGF治疗减少了亡标志物,增加了扩散和血管功能蛋白质表达.
- bFGF有效地降低了关键内细胞网膜应激 (ERS) 蛋白和基因的表达.
结论:
- 在糖尿病条件下,bFGF对HUVEC细胞具有保护作用.
- bFGF通过减轻内分泌网膜应激 (ERS) 来改善内皮细胞功能.
- bFGF代表了一种有前途的治疗剂,用于治疗糖尿病血管病变.
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