主体因素和侵入性脑膜炎球菌对细菌粘附,增殖,主要鼻上皮质屏障功能和免疫反应的影响
Daan W Arends1,2, Debbie van Rooijen1, Esther van Woudenbergh1,2
1Center for Immunology of Infectious Diseases and Vaccines, National Institute for Public Health and the Environment, RIVM, Bilthoven, the Netherlands.
Microbiology spectrum
|June 30, 2025
概括
主体-病原体相互作用决定了侵入性脑膜炎球菌病 (IMD) 的风险. 特定的Neisseria meningitidis菌株和个别的鼻上皮细胞具有独特的相互作用,影响疾病的严重程度和潜在的爆发.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 皮质生物学 皮质生物学
背景情况:
- 脑膜炎Neisseria导致侵入性脑膜炎球菌疾病 (IMD),某些超侵入性血统,如克隆复合体11 (cc11) 构成重大公共卫生威胁.
- 了解最初的殖民和上皮屏障相互作用对于识别导致脑膜炎球菌过度入侵的因素至关重要.
研究的目的:
- 研究Neisseria meningitidis在人类第一鼻上皮细胞的殖民中的宿主和菌株依赖差异.
- 阐明这些相互作用如何影响表皮屏障完整性和宿主免疫反应,可能解释超侵入性.
主要方法:
- 使用来自多个捐赠者的空气-液体接口 (ALI) 区分的初级鼻上皮细胞.
- 感染细胞与参考和超侵入性cc11尼塞利亚脑膜炎菌株 (血清组C,W和未封装).
- 评估过体电阻 (TEER),细菌结合/生长以及上皮细胞因子的产生 (CCL20,CXCL1,CXCL8,CXCL10,IL-18).
主要成果:
- 细菌负载和TEER变化取决于供体和菌株,较高的负载与上皮质透性增加相关.
- 超侵袭性cc11菌株表现出明显的结合模式,MenW cc11比其参考对应物具有更高的结合性.
- 脑膜炎球菌感染普遍诱导特定的细胞因子,CCL20在MenW cc11感染后升高;然而,细胞因子配置主要由上皮细胞供体驱动.
结论:
- 尼塞利亚脑膜炎上皮质感染的结果受到特定宿主-病原体相互作用的显著影响.
- 这些供体菌株特异性动态对于确定疾病进展和IMD的潜力至关重要.
- 了解这些相互作用可以帮助识别有IMD风险的个体,并预测未来的潜在爆发菌株.
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