相关实验视频

Updated: Sep 17, 2025

Assays for Validating Histone Acetyltransferase Inhibitors

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

棕酸诱导HDACs,神经细胞染色体乙化和基因表达的动态时间依赖的变化

Saúl Santiago Rueda-Díaz¹, Iker Francisco Soto-Santarriaga^1,2, Karla Torres-Arciga³

¹Departamento de Medicina Genómica y Toxicología Ambiental. Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM), AP 70-228, Ciudad de México, 04510, México.

Neurochemical research

|June 30, 2025

概括

富含棕酸 (PA) 的高脂肪饮食会影响大脑健康. 这项研究揭示了PA改变神经元中的表观遗传机制,影响与认知功能相关的基因表达.

科学领域:

神经科学是一个神经科学.
代谢性疾病研究研究
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

高脂肪饮食 (HFD) 与代谢疾病和认知衰退有关.
棕酸 (PA) 是HFD的一个关键成分,它会影响外围组织和大脑功能,包括胰岛素抵抗和基因表达.
尽管已知对PPAR-γ和PGC-α等转录因子的影响,但PA对神经元表观遗传机制的影响仍未得到充分研究.

研究的目的:

研究棕酸 (PA) 对人类神经母细胞瘤细胞内表观遗传机制的影响.
为了确定PA诱导的基因脱乙酶 (HDACs),染色质乙化和基因表达的变化.
探索PA,表观遗传修饰和BDNF基因和LINE1逆转移素的表达之间的关系.

主要方法:

利用差异化的人类神经母细胞瘤细胞.
分析了I类组素脱乙酶 (HDACs) 含量的变化.
评估的染色质乙化水平.
测量了BDNF基因和LINE1逆转移素的差异表达.

主要成果:

鉴定了与棕酸 (PA) 相关的I类组胺脱甲基酶 (HDACs) 的变化.
观察到染色质乙化模式的变化.
发现了来自大脑的神经营养因子 (BDNF) 基因的差异表达.
检测到非编码逆转移素的改变表达,LINE1.1.

结论:

棕酸 (PA) 影响神经元中的表观遗传调节.
在HDAC和染色体乙化中PA诱导的变化影响了关键神经元基因如BDNF和LINE1.1的表达.
这些发现提供了关于高脂肪饮食如何通过表观遗传修饰导致神经功能障碍的见解.

关键词:

这就是为什么BDNF是BDNF.这就是HDACs.高脂肪饮食高脂肪饮食基斯乙化基斯乙化棕酸是一种棕酸.

更多相关视频

Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue

Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue

Published on: November 30, 2018

Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange ABE

Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange ABE

Published on: February 18, 2013

相关概念视频

Histone Modification

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...

Spreading of Chromatin Modifications

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...

Chromatin Modification in iPS Cells

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...

Covalently Linked Protein Regulators

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....

Euchromatin

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...

Heterochromatin

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...