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Updated: Sep 17, 2025

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
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Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

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在神经元生长和轴突中可视化纳米结构,使用超分辨率结构化照明显微镜

Motohiro Nozumi1, Michihiro Igarashi2

  • 1Department of Molecular Cell Biology, School of Medicine, and Graduate School of Medical and Dental Sciences, Niigata University, 1-757 Asahimachi-Dori, Chuo-Ku, Niigata, 951-8510, Japan. mnozumi@med.niigata-u.ac.jp.

Anatomical science international
|June 30, 2025
PubMed
概括

结构化照明显微镜 (SIM) 允许详细观察神经元结构. 这项研究详细介绍了用于可视化生长和轴突的SIM方法,揭示了轴突内的酸化GAP-43定位.

关键词:
亚克森人是一个亚克森人.在GAP-43中.增长的形形.与膜相关的周期性骨架结构化照明显微镜 (SIM) 的使用

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Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy14:11

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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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