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在非阻塞性亚精中与自相关的生物标志物:从转录组学和单细胞测序的见解

Juncheng Yao¹, Yuanyuan Zhang¹, Tingting Lan²

¹Department of Assisted Reproduction Center, Sichuan Provincial People's Hospital, Chengdu, Sichuan, 610041, China.

Journal of assisted reproduction and genetics

|June 30, 2025

概括

研究人员确定了四个与自相关的基因 (ARG) 作为非阻断性亚精细菌 (NOA) 的潜在生物标志物. 这些发现可能会导致新的诊断工具和治疗男性不孕症的方法.

科学领域:

生殖生物学和遗传学生殖生物学和遗传学
细胞生物学和分子机制

背景情况:

自会影响精子细胞的功能,可能在非阻塞性精子缺血症 (NOA) 中起作用.
自相关基因 (ARG) 在NOA病理生理学中的特定参与仍然在很大程度上未经调查.

研究的目的:

识别和验证非阻塞性亚子精子症 (NOA) 的新型自相关基因生物标志物.
使用转录基因数据探索与NOA相关的潜在分子和免疫机制.

主要方法:

差异基因表达分析,加权基因共同表达网络分析 (WGCNA) 和机器学习算法应用于NOA转录数据集.
使用ROC曲线评估生物标志物的表现,并通过丰富,免疫和单细胞分析获得机理性见解.
使用独立数据集和RT-qPCR进行了验证.

主要成果:

四个基因 (ATP6V1E2,UBQLN2,FYCO1和ITPR1) 被确定为NOA的显著生物标志物,具有很高的诊断准确性.
FYCO1表达与特定的免疫细胞群 (T毛囊辅助细胞和调节性T细胞) 相关联.
单细胞分析表明,这些生物标志物的表达模式在丸配体和光滑肌肉细胞中是不同的.

结论:

已识别的ARG (ATP6V1E2,UBQLN2,FYCO1,ITPR1) 是NOA的诊断生物标志物,有望成为NOA的诊断生物标志物.
这些生物标志物可能提供潜在的治疗点,为NOA的新型治疗策略铺平了道路.

关键词:

自自是一种自的过程.诊断诊断诊断诊断诊断豁免权是一种豁免权.非阻塞性阿佐精子症.反转录聚合酶连锁反应的逆转录.

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