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聚乙烯 - 糖醇 - 结合合合体与可调整大小的合体,用于细胞内mRNA输送

Yue Sun¹, Xi Wu¹, Kimberle Shen²

¹Center for Sustainable Materials (SusMat), School of Materials Science and Engineering, Nanyang Technological University, 639798 Singapore.

|June 30, 2025

概括

研究人员使用PEGylated来开发纳米级的协同化纳米滴 (CNs),以提高稳定性和尺寸控制. 这些CNs在生理温度下显示出增强的细胞吸收和mRNA递送,扩大了基于共体的系统的应用.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
化学工程是化学工程的重要组成部分.

背景情况:

液-液相分离 (LLPS) 产生的同微滴 (CM) 对生物医学用途具有前景,但面临尺寸和稳定性的挑战.
现有的CM通常呈现微米大小和凝聚,限制其在细胞内输送或微催化等应用中的实用性.

研究的目的:

使用聚乙烯糖醇 (PEG) 结合来控制基于的协同体的尺寸和稳定性.
恢复和增强细胞吸收和mRNA传递能力这些修饰的同体 (CNs).

主要方法:

结合胺丰富的皮 (HBpep) 与PEG,产生PEG化HBpep.
准备PEGylated和非PEGylatedHBpep的混合物,以形成协同化纳米滴 (CNs).
修改带有正电荷的序列,以改善mRNA招募和细胞递送.

主要成果:

在纳米尺度范围内,PEGylation稳定了协同,其尺寸分布基于PEGylated-to-non-PEGylated的比例而受到控制.
整合正电荷恢复了mRNA招募和CNs的细胞内传递功能.
经PEG稳定的CNs在37°C时表现出增强的细胞吸收和mRNA转染效率.

结论:

这一策略有效地控制了同胞体的大小和稳定性,创造了功能性的纳米级同胞体纳米滴 (CNs).
开发的CNs提供了改善的细胞吸收和mRNA递送,适合生理条件.
这种方法为设计基于LLPS的先进交付系统提供了一个多功能平台,用于体内应用,催化和生物反应器.

关键词:

提供mRNA的交付.类协同化物.聚合物 - 酸联合体尺寸稳定性尺寸稳定性

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