Jove
Visualize
联系我们

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Immune microenvironment and noncoding RNA shape early colorectal carcinogenesis in patients with premalignant lesions.

Science translational medicine·2026
Same author

The American Cancer Society National Lung Cancer Roundtable strategic plan: Challenges in clinical guideline recommendations for biomarker testing in lung cancer.

Cancer·2026
Same author

Evidence-based team intervention to reduce diagnostic errors in anaemia and CKD diagnoses in primary care: protocol for a stepped-wedge cluster RCT.

BMJ open·2026
Same author

AI-powered spatial cell phenomics enhances risk stratification in non-small cell lung cancer.

Nature communications·2025
Same author

Bridging the Evidence Gap: Perioperative Implications of the 2025 ACC/AHA Antithrombotic Guideline for ACS.

Journal of cardiothoracic and vascular anesthesia·2025
Same author

Surgical advocacy as an academic career path: A guide for promotion discussions.

Surgery·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Sep 17, 2025

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research
09:12

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

Published on: June 10, 2025

432

高通量自动化多重复合免疫光检测用于翻译研究.

Kevin Hwang1, Alex Veith1, Lauren Duro1

  • 1Ultivue, Inc.

Journal of visualized experiments : JoVE
|June 30, 2025
PubMed
概括

一种新的多重免疫光技术 (mIF) 使用DNA条形编码抗体进行高度敏感,高通量组织分析. 这种先进的mIF方法克服了生物标志物发现的灵敏度和吞吐量限制.

科学领域:

  • 生物医学成像技术 生物医学成像技术
  • 分子病理学分子病理学
  • 免疫组织化学 免疫组织化学

背景情况:

  • 多重免疫光学 (mIF) 能够在单个组织段中对多个蛋白质生物标记物的空间分析.
  • 现有的mIF技术往往会损害灵敏度或吞吐量,阻碍低丰度生物标志物的检测.
  • 这种限制对于对复杂的生物样本进行全面分析至关重要.

研究的目的:

  • 引入一种新的mIF方法,克服灵敏度-通量权衡.
  • 为了实现高质量,高复杂化和高通量组织分析.
  • 为了促进多种生物标志物的检测,以获得更深入的生物学见解.

主要方法:

  • 利用与抗体结合的DNA条形码通过单分子放大来进行放大.
  • 采用光标记的寡核酸的连续检测周期来可视化生物标志物.
  • 集成的自动组织准备,信号放大,代成像和AI增强的空间分析.
  • 将该协议应用于甲固定,嵌 (FFPE) 多组织微阵列 (TMA).

主要成果:

  • 获得了与临床级免疫组织化学 (IHC) 相当的染色质量.
  • 每个样本可检测8个或更多的生物标志物,并具有高复杂化.

更多相关视频

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens
10:49

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

Published on: January 21, 2019

20.8K
Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
08:05

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Published on: December 12, 2011

28.5K

相关实验视频

Last Updated: Sep 17, 2025

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research
09:12

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

Published on: June 10, 2025

432
Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens
10:49

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

Published on: January 21, 2019

20.8K
Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
08:05

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Published on: December 12, 2011

28.5K
  • 展示了高通量全幻灯片成像能力.
  • 成功应用于FFPE TMA,包括桃体,黑色素瘤,结肠和淋巴结组织.

    • 结论:

    • 开发的DNA条形编码mIF试验克服了灵敏度,复杂化和吞吐量的关键限制.
    • 这种先进的技术为瘤微环境和免疫格局提供了更深入的见解.
    • 促进对分子特征和治疗反应生物标志物的理解,推进精准医学.