近红外化探头的分子优化,用于在急性肝损伤中对聚硫化物进行成像
Zheng Wen1,2, Wei Peng1,2, Yongzhi Qi1
1Key Laboratory of Haikou Trauma, Key Laboratory of Hainan Trauma and Disaster Rescue, Key Laboratory of Emergency and Trauma of Ministry of Education, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou 571199, China.
Analytical chemistry
|June 30, 2025
概括
研究人员开发了一种新的近红外光探头DCICl-H2Sn,以实时检测多硫化 (H2Sn). 这项探测表明,较高的H2Sn水平与小鼠肝损伤严重程度的降低相关,这表明H2Sn是急性肝损伤的潜在生物标志物.
科学领域:
- 生物化学 生物化学
- 化学生物学 化学生物学
- 分子生物学分子生物学
背景情况:
- 聚硫化 (H2Sn) 是关键的反应硫物种,参与细胞信号传递,氧化应激和细胞死亡.
- 实时体内检测H2Sn对于理解其生理作用至关重要,但由于其不稳定性而具有挑战性.
研究的目的:
- 开发和验证近红外光 (NIRF) 探头,用于在生物系统中敏感和特定检测H2Sn.
- 研究H2Sn在急性肝损伤 (ALI) 模型中的作用及其作为损伤严重程度生物标志物的潜力.
主要方法:
- 一个新的NIRF探测器DCICl-H2Sn的选和描述,该探测器基于二异索支架.
- 使用探头实时监测活细胞和ALI小鼠模型中的外源和内源H2Sn水平.
- 评估H2Sn水平与由乙氨基 (APAP) 或四化碳 (CCl4) 诱导的肝损伤严重程度之间的相关性.
主要成果:
- DCICl-H2Sn探头在与H2Sn反应时,在675nm处表现出"启动"光反应,显示出高特异性和优良的光物理性质.
- 在体内研究表明,H2Sn水平与APAP或CCl4诱导的ALI严重程度之间存在负相关性.
- 施用西利宾 (SLB) 减少了肝损伤,同时增加了H2Sn水平.
结论:
- DCICl-H2Sn探头是实时检测和动态监测H2Sn在体外和体外的有效工具.
- H2Sn水平可以作为评估急性肝损伤严重程度的潜在生物标志物.
- 这些发现突显了H2Sn在肝损伤中的保护作用,并提供了对其分子机制的见解.
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