Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

The Supercomplexes in the Crista Membrane01:41

The Supercomplexes in the Crista Membrane

2.6K
The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
2.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Sex- and stressor-dependent effects of metformin on cardiac fibrosis in plasminogen activator inhibitor-1 deficiency.

Journal of molecular and cellular cardiology·2026
Same author

Unbiased distance correlation with sample-size-aware confidence bounds for comparative omics network analysis.

Frontiers in bioinformatics·2026
Same author

Pairwise causal discovery in biochemical networks: A survey on directionality inference within complex networks from stationary observations.

PloS one·2026
Same author

Persistent organic pollutant concentrations in human pancreas correlate with markers of beta cell dysfunction.

Nature communications·2026
Same author

Extracellular Vesicles Modulation by an Adiponectin Receptor Agonist Provides Cardioprotection for Myocardial Ischemic Injury.

Advanced healthcare materials·2026
Same author

Functional and metabolomic analyses of brown adipose tissue during cold-deacclimation reveal rapid N-acetylated amino acid adaptations.

iScience·2026

相关实验视频

Updated: Sep 17, 2025

Author Spotlight: Unveiling Oxidative Phosphorylation System Dynamics and Mitochondrial Roles in Health and Disease
05:45

Author Spotlight: Unveiling Oxidative Phosphorylation System Dynamics and Mitochondrial Roles in Health and Disease

Published on: May 3, 2024

1.4K

复合体分析识别了小鼠心力衰竭中线粒体超级复合体的变化.

Claire Fong-McMaster1,2,3, Ella McIlroy1,2, Michelle M Levesque1,2,3

  • 1Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.

American journal of physiology. Heart and circulatory physiology
|June 30, 2025
PubMed
概括

心力衰竭改变了线粒体超级复合体,影响了代谢功能. 这项研究揭示了这些复合体内的蛋白质丰度的变化,为心力衰竭机制提供了新的见解.

关键词:
蓝色原生页面 页面心肌病性心脏病 - 心肌病性心脏病质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量氧化酸化是一种氧化酸化.一些呼吸,一些呼吸.

更多相关视频

Analyzing Supercomplexes of the Mitochondrial Electron Transport Chain with Native Electrophoresis, In-gel Assays, and Electroelution
08:37

Analyzing Supercomplexes of the Mitochondrial Electron Transport Chain with Native Electrophoresis, In-gel Assays, and Electroelution

Published on: June 1, 2017

14.3K
Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes
08:04

Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes

Published on: August 16, 2021

2.7K

相关实验视频

Last Updated: Sep 17, 2025

Author Spotlight: Unveiling Oxidative Phosphorylation System Dynamics and Mitochondrial Roles in Health and Disease
05:45

Author Spotlight: Unveiling Oxidative Phosphorylation System Dynamics and Mitochondrial Roles in Health and Disease

Published on: May 3, 2024

1.4K
Analyzing Supercomplexes of the Mitochondrial Electron Transport Chain with Native Electrophoresis, In-gel Assays, and Electroelution
08:37

Analyzing Supercomplexes of the Mitochondrial Electron Transport Chain with Native Electrophoresis, In-gel Assays, and Electroelution

Published on: June 1, 2017

14.3K
Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes
08:04

Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes

Published on: August 16, 2021

2.7K

科学领域:

  • 生物化学 生化学
  • 心脏病学 心脏病学
  • 线粒体生物学 线粒体生物学

背景情况:

  • 心力衰竭与代谢功能障碍和线粒体活动受损有关.
  • 线粒体超级复合体对于电子运输链效率至关重要,在心力衰竭中减少.

研究的目的:

  • 在心力衰竭模型中研究线粒体超级复合体的蛋白质组合.
  • 为了确定有助于心力衰竭中代谢障碍的特定蛋白质变化.

主要方法:

  • 利用心肌梗塞 (MI) 的小鼠模型来模拟心力衰竭.
  • 采用复合体分析,结合蓝色原生PAGE和质谱,分析线粒体超级复合体.
  • 评估心脏功能使用二维心声回声学.

主要成果:

  • 在MI小鼠的线粒体超级复合体内确定了电子运输链子单元的相对丰度变化.
  • 观察到参与alpha-ketoglutarate脱酶代谢的蛋白质水平下降,包括二利胺S-succinyltransferase (DLST).
  • 检测到氧化还原蛋白如超氧化脱酶2 (SOD2) 的丰度降低,以及MI线粒体中的核糖体蛋白的变化.

结论:

  • 这项研究揭示了心力衰竭期间线粒体超级复杂蛋白质组的显著变化.
  • 这些发现为心力衰竭中代谢功能障碍背后的分子机制提供了新的见解.
  • 超级复合体的蛋白质组成变化可能是心力衰竭进展的关键因素.