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选择针对重组甲状腺刺激激素受体蛋白的不同表位的aptamers

Jiajie Xu^1,2,3, Ying Luo¹, Zhenhao Long⁴

¹Otolaryngology & Head and Neck Center, Cancer Center, Department of Head and Neck Surgery, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou, Zhejiang 310014, China.

Analytical chemistry

|June 30, 2025

概括

研究人员开发了一种新的aptamer选择方法来识别向蛋白质特定部分的分子,从而推进甲状腺癌的诊断和治疗方法.

科学领域:

生物化学生物化学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

在生物标志物发现,疫苗开发和精准医学中的向疗法中,皮质的识别至关重要.
通过SELEX生成的aptamers是有价值的识别元素,但在具有挑战性的目标上选择它们作为本地表位是很困难的.
原生折叠的蛋白质制剂很少,阻碍了对特定表位体的体的发展.

研究的目的:

开发一种新的SELEX方法,用于产生针对目标蛋白质多个表位体的aptamer.
识别针对甲状腺刺激激素受体 (TSHR) 不同表位体的aptamer.
为了验证这些aptamers在甲状腺癌病理学中的诊断潜力.

主要方法:

开发了一种新的SELEX方法,用于产生针对TSHR的体.
使用Epitope-I特异性受体来固定TSHR,从而可以选择Epitope-II特异性受体.
在细胞和组织水平上使用流动细胞计,免疫光和免疫组织化学测试了aptamer的特异性和亲和力.

主要成果:

成功识别了针对TSHR蛋白质的独特表位体的新型吸收体.
开发的阿普坦体对TSHR结合具有很高的特异性和亲和力.
免疫组织化学证实了阿普坦体在识别TSHR阳性甲状腺瘤的诊断实用性.

结论:

这项研究提出了一种可通用的方法,用于开发针对多种蛋白质表位的体.
已识别的TSHR亚体显示出作为甲状腺癌诊断和治疗的分子探针的前景.
这种方法加速了对各种疾病的生物标志物和治疗点的发现.

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