转录组的参考导向检测揭示了循环RNA相关的竞争性内源RNA网络,以应对猪肺中的三个呼吸病原体
Qian Wu1,2, Kun Du1,2, Mao-Qing Gu1,2
1Animal Disease Prevention and Control and Healthy Breeding Engineering Technology Research Centre, Mianyang Normal University, Mianyang, 621000, China.
Virology journal
|June 30, 2025
概括
这项研究揭示了循环RNAs (circRNAs) 如何对主要猪肺病原体如PRRSV,ASFV和Mhp作出反应. 它确定了参与免疫反应和肺损伤机制的关键circRNA网络.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 猪生殖和呼吸系统综合征病毒 (PRRSV),非洲猪瘟病毒 (ASFV) 和Mycoplasma hyopneumoniae (Mhp) 在全球猪业中造成严重的肺损伤和经济损失.
- 已知循环RNAs (circRNAs) 调节免疫反应和病毒感染,但它们在这些病原体引起的猪肺感染中的特定作用基本上是未知的.
研究的目的:
- 在感染PRRSV,ASFV和MHP的猪肺中识别和描述circRNA及其竞争的内源RNA (ceRNA) 网络.
- 研究circRNAs在对这三个主要呼吸道病原体的反应中的差异表达和功能作用.
主要方法:
- 在91个猪肺数据集上利用RNA测序 (RNA-seq) 来进行参考引导的转录组重建和circRNA检测.
- 使用生物信息学方法构建与circRNA相关的ceRNA网络.
- 对差异表达的circRNA进行了功能注释和路径丰富分析.
主要成果:
- 在猪肺中鉴定了72,203个已知的和14,344个新的circRNAs.
- 在响应PRRSV,ASFV和MHP时分别发现66,67和197个差异表达的circRNA.
- 发现Mhp和ASFV上调的circRNA ceRNA网络分别富含幽默免疫和活性氧物种通路,而PRRSV下调的cirRNA与RNA剪接有关,在PRRSV感染后观察到的循环比率下降.
结论:
- 提供了猪肺中circRNAs的全面目录,并突出了它们对PRRSV,ASFV和Mhp的独特反应.
- 阐明了circRNA ceRNA网络在免疫反应和受这些病原体影响的细胞通路中的特定功能作用.
- 建议 circRNA循环比率的减少可能是PRRSV感染期间的关键机制,为肺损伤机制提供了潜在的见解.
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