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线粒体酸5通过减轻线粒体功能障碍来提高野猪精子质量.

Ruyuan Wang1, Adedeji O Adetunji2, Lingjiang Min1

  • 1College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.

Frontiers in cell and developmental biology
|July 1, 2025
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概括

甲基酸5 (MA-5) 改善了野猪精子质量和线粒体功能,长达2小时. 将MA-5与pyrroloquinoline quinone (PQQ) 结合在一起,对于保持高质量的精子长达4小时至关重要,有助于维护野猪精子和人工授精.

关键词:
野猪的精子来自于野猪的精子代谢过程中的代谢.线粒体中的线粒体.米托康酸 5 5 米托康酸质量质量质量质量质量质量.

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科学领域:

  • 生殖生物学 生殖生物学
  • 线粒体生物学 线粒体生物学
  • 生物化学 生物化学

背景情况:

  • 线粒体功能障碍增加了氧化应激,并耗尽了ATP.
  • 已知Mitochonic acid 5 (MA-5) 可以改善线粒体新陈代谢和ATP的产生.
  • 优化野猪精子保存对于人工授精 (AI) 成功至关重要.

研究的目的:

  • 研究不同度的MA-5对野猪精子运动,形态和线粒体功能的影响.
  • 为了确定MA-5在野猪精子扩展器中的最佳度和潜伏时间.
  • 为了评估MA-5和罗基诺林 (PQQ) 对长期精子保存的联合作用.

主要方法:

  • 猪精子在BTS扩展器中以不同度的MA-5 (0.01,0.1,1和10nM) 进行化.
  • 评估了精子运动性,渐进性运动性,膜完整性和体完整性.
  • 分析了线粒体膜潜力,ATP含量,ROS水平以及基因/蛋白质表达 (MT-ND1,MT-ND6,TFAM).

主要成果:

  • 1 nM MA-5显著增强精子运动性和渐进性运动性,长达2小时.
  • 2小时后,MA-5 (1nM) 增加了线粒体膜潜力和ATP含量.
  • 将MA-5与10nM PQQ结合使用,在化4小时后恢复了线粒体功能,改善了精子质量.

结论:

  • MA-5 (1nM) 在37°C下有效维持野猪精子质量和线粒体代谢,长达2小时.
  • MA-5和PQQ的组合对于保持高质量的野猪精子长达4小时至关重要.
  • 通过保持精子质量和线粒体功能,MA-5和PQQ显示了改善野猪精子保存和AI实践的潜力.