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Updated: Sep 17, 2025

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

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使用CRISPR/Cas9在斑点木中产生高效的生殖基因突变Pararge aegeria

Anna B Shoshan1,2, Kalle Tunström1,3, Christopher W Wheat1

  • 1Department of Zoology Stockholm University Stockholm Sweden.

Ecology and evolution
|July 1, 2025
PubMed
概括

研究人员开发了一种高效的CRISPR/Cas9方法,用于在斑点木中产生生殖系突变,从而推进对生态模型中不可见的特征的基因型-表型研究.

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Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner06:37

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Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas907:45

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9

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相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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