循环蛋白质组面板用于非侵入性诊断和预测血栓血管炎的Obliterans
Gang Fang1, Yuan Fang1, Lutong Yan1
1Department of Vascular Surgery, Zhongshan Hospital, Institute of Vascular Surgery, Fudan University, National Clinical Research Center for Interventional Medicine, Shanghai 200032, China.
Journal of proteome research
|July 1, 2025
概括
血液中的生物标志物可以帮助更早地诊断出 Thromboangiitis obliterans (TAO),并预测疾病的严重程度. 这项研究确定了蛋白质特征,以改善TAO患者的早期检测和风险分层.
科学领域:
- 蛋白质组学是指蛋白质组学.
- 生物标志物发现发现
- 血管医学 血管医学
背景情况:
- 阻塞性血管炎 (TAO) 带来了诊断挑战,往往导致晚期检测和高截肢率.
- 对于特定于TAO的早期诊断和疾病发病阶段生物标志物存在关键需求.
研究的目的:
- 识别和验证血清蛋白生物标记物,用于早期诊断和风险分层的Thromboangiitis obliterans (TAO).
- 开发预测模型,以区分TAO患者与健康对照者,以及活跃与不活跃的疾病阶段.
主要方法:
- 一个基于质谱 (MS) 的分阶段工作流程,涉及发现 (DIA-MS),验证 (目标 PRM) 和验证 (ELISA) 队列.
- 从健康对照组 (HCs) 和TAO患者的血清样本进行定量蛋白质组分析.
- 机器学习算法被用来构建预测生物标志物面板.
主要成果:
- 在发现队列中确定了842种蛋白质,量化了470种蛋白质.
- 开发了9蛋白和7蛋白血清生物标记面板,能够区分TAO与HC,以及活跃TAO与非活跃TAO.
- 一个综合预后小组证明了活跃TAO患者的有效风险分层,通过前ELISA分析进行验证.
结论:
- 这项研究提出了TAO的综合血清蛋白质组景观,提供了新的生物学见解.
- 鉴定出的血清蛋白签名显示了改善TAO早期诊断和风险分层的显著潜力.
- 经过验证的蛋白质组面板为TAO管理中的临床应用提供了稳定和强大的方法.
关键词:
生物标志物 生物标志物质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量蛋白质的签名 蛋白质的签名血清蛋白质组概况分析血栓血管炎的消灭者更多相关视频
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