单克隆抗体和非单克隆表面活性剂在空气-水界面的竞争性吸附
Benjamin R Thompson1, Kiet G Pham1, Minh D Phan2
1Department of Chemical & Biomolecular Engineering, Center for Neutron Science, University of Delaware, Newark, Delaware 19716, United States.
ACS applied materials & interfaces
|July 1, 2025
概括
单克隆抗体 (mAbs) 在接口上吸附,形成影响药物稳定的粒子. 像Poloxamer 188这样的表面活性剂可以减轻这种情况,但它们的添加顺序会影响mAb相互作用和配方稳定性.
科学领域:
- 蛋白质配方科学 蛋白质配方科学
- 生物物理化学 生物物理化学
- 材料科学是一种材料科学.
背景情况:
- 单克隆抗体 (mAbs) 在空气-水接口上吸附,导致粒子形成和治疗效率降低.
- 吸附的mAbs的界面切割模量与长期配方稳定性相关,为加速测试提供了潜力.
- 辅助剂,特别是非离子表面活性剂,用于防止mAb吸附并减轻界面诱导的不稳定性.
研究的目的:
- 为了研究mAbs在空气-水界面上的分子相互作用,使用和不使用Poloxamer 188 (P188).
- 了解机械变形和辅助剂的存在如何影响mAb吸附和界面特性.
- 提供分子层面的洞察力,了解空气-水界面对治疗配方mAb稳定性的影响.
主要方法:
- 界面压力和风湿学测量.
- 布鲁斯特角显微镜 (BAM) 用于表面可视化.
- 中子反射计 (NR) 用于量化压力下的分子层变化.
主要成果:
- mAbs 吸附速度很快,但表面压力和模块在吸附后形成平原.
- NR揭示了在扩张应激下分子层的变化.
- 添加P188增加了表面压力和降低了模块,改变了mAb表面拓.
- 添加顺序 (预先注入与分相注入) 显著影响了mAb-P188相互作用.
结论:
- 空气-水界面显著影响mAb的相互作用和稳定性.
- 波洛克萨默188修改了mAb的界面行为,但mAbs仍然被吸附.
- 了解辅助剂添加的顺序对于优化治疗配方和防止mAb不稳定性至关重要.
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