使用Saccharomyces cerevisiae幽灵的蒙纳斯克红色素的微封装:过程优化和表征
Mohammed S Khalil1, Shaimaa O Makled2, Nefertiti El-Nikhely1,3
1Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt.
Probiotics and antimicrobial proteins
|July 1, 2025
概括
蒙纳斯红色素 (MRP) 用酵母细胞幽灵 (ScGs) 进行微封装,以增强药物输送. 这种新的生物配方改善了MRP.
科学领域:
- 生物材料科学 生物材料科学
- 微生物学 微生物学
- 药物输送系统 药物输送系统
背景情况:
- 莫纳斯红色素 (MRP) 具有治疗潜力,但需要改进的输送方法.
- 微生物的二次代谢产物为新型治疗剂提供了机会.
- 麦芽菌幽灵 (Saccharomyces cerevisiae ghosts,ScGs) 是一个可行的生物载体,用于封装生物活性化合物.
研究的目的:
- 开发和优化使用Saccharomyces cerevisiae幽灵 (ScGs) 对Monascus红色素 (MRP) 的微封装技术.
- 描述由此产生的MRP-ScGs生物配方,并评估其药物释放特征.
- 评估微封装MRP的增强生物活性和细胞吸收,以潜在的生物医学应用.
主要方法:
- 在S. cerevisiae幽灵中进行MRP的微封装.
- 使用光,电子和共聚焦显微镜进行形态表征.
- 使用盒子-贝恩肯设计 (BBD) 和响应表面方法 (RSM) 优化微封装参数.
- 在酸盐缓冲盐水 (PBS) 中使用Tween 80进行药物释放研究.
- 对A549肺癌细胞的细胞毒性测定.
- 在基生物信息分析用于目标预测.
主要成果:
- 成功生成了具有保存结构和适合微封装的内部体积的S. cerevisiae幽灵 (ScGs).
- 在特定条件下 (25°C,300 mg/mL MRP,125rpm,90分钟) 的负载效率为61.4%.
- 微封装MRP (MRP-ScGs) 与自由MRP相比,表现出双相持续释放特征和对A549肺癌细胞的增强细胞毒性.
- 对焦显微镜证实了MRP-ScG的细胞吸收增加,与增强的生物活性相关.
结论:
- MRP-ScGs代表生物医学应用的有前途的生物微囊平台,提供结构稳定性.
- 微封装过程显著提高了Monascus红色素的治疗潜力和细胞吸收.
- 在基分析表明,MRP-ScGs的向药物输送应用有潜力.
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