关于血液成分导致基于aptamer的电化学生物传感器的漂移
Joshua D Pham1,2, Lisa C Fetter1,3, Julian Gerson1,4
1Center for Bioengineering, University of California Santa Barbara, Santa Barbara, California 93106, United States.
ACS sensors
|July 1, 2025
概括
基于电化学体 (EAB) 的传感器由于大蛋白质而在血液中经历信号漂移. 识别这些高分子量蛋白 (>100 kDa) 是改善长期体内传感器性能的关键.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 生物技术是生物技术.
背景情况:
- 基于电化学体 (EAB) 的传感器可以实时进行体内分子监测.
- 信号漂移,随着时间的推移减少传感器输出,阻碍了长期EAB传感器的部署.
- 之前的研究将漂移归因于血液中的aptamer污染和酶降解.
研究的目的:
- 为了研究负责体外EAB传感器信号漂移的特定血液成分.
- 为了确定导致EAB传感器漂移的血液成分的分子特征.
主要方法:
- 在全血,洗血细胞和血中对EAB传感器漂移的比较分析.
- 使用大小分割的血清和血样本进行漂移的评估.
- 蛋白质分子重量影响传感器漂移的评估.
主要成果:
- 在体外,EAB传感器漂移是由血液蛋白质引起的,而不是血液细胞.
- 诱导漂移的蛋白质的分子量大于100kDa.
- 这一发现支持了以前使用分子重量选择性膜来减少漂移的策略.
结论:
- 血液蛋白质,特别是100kDa以上的蛋白质,是体外EAB传感器信号漂移的主要原因.
- 了解这些蛋白相互作用对于开发更稳定,更可靠的体内EAB传感器至关重要.
- 这项研究为工程改进的传感器设计和长期生物医学应用的表面修改提供了基础.
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